Institute for Research on Cancer and Aging in Nice (IRCAN), Université Côte d'Azur, CNRS, INSERM, Nice, France.
Institut Fédératif de Recherche-Ressources Marines (IFR MARRES), Université Côte d'Azur, Nice, France.
Methods Mol Biol. 2022;2450:649-662. doi: 10.1007/978-1-0716-2172-1_35.
The sea anemone Nematostella vectensis has emerged as a powerful research model to understand at the gene regulatory network level, to what extend regeneration recapitulates embryonic development. Such comparison involves massive transcriptomic analysis, a routine approach for identifying differential gene expression. Here we present a workflow to build a user-friendly, mineable, and open-access database providing access to the scientific community to various RNAseq datasets.
海葵 Nematostella vectensis 已成为一种强大的研究模型，可以在基因调控网络水平上理解再生在多大程度上重现胚胎发育。这种比较涉及大规模的转录组分析，这是一种常规的方法，用于鉴定差异基因表达。在这里，我们提出了一种工作流程，用于构建一个用户友好、可挖掘和开放访问的数据库，为科学界提供各种 RNAseq 数据集的访问权限。
