创建一个用户友好且开放获取的基因表达数据库，用于比较 Nematostella vectensis 胚胎发育和再生。
Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostella vectensis.
机构信息
Institute for Research on Cancer and Aging in Nice (IRCAN), Université Côte d'Azur, CNRS, INSERM, Nice, France.
Institut Fédératif de Recherche-Ressources Marines (IFR MARRES), Université Côte d'Azur, Nice, France.
出版信息
Methods Mol Biol. 2022;2450:649-662. doi: 10.1007/978-1-0716-2172-1_35.
Abstract
The sea anemone Nematostella vectensis has emerged as a powerful research model to understand at the gene regulatory network level, to what extend regeneration recapitulates embryonic development. Such comparison involves massive transcriptomic analysis, a routine approach for identifying differential gene expression. Here we present a workflow to build a user-friendly, mineable, and open-access database providing access to the scientific community to various RNAseq datasets.
摘要
海葵 Nematostella vectensis 已成为一种强大的研究模型，可以在基因调控网络水平上理解再生在多大程度上重现胚胎发育。这种比较涉及大规模的转录组分析，这是一种常规的方法，用于鉴定差异基因表达。在这里，我们提出了一种工作流程，用于构建一个用户友好、可挖掘和开放访问的数据库，为科学界提供各种 RNAseq 数据集的访问权限。
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