University of Puerto Rico, San Juan, Puerto Rico.
Methods Mol Biol. 2021;2219:241-252. doi: 10.1007/978-1-0716-0974-3_15.
Functional studies on echinoderms have been reduced to the use of pharmacological treatments. The ability to modulate the genetic expression of regenerating tissues can elucidate potential effectors during this process. Here we describe an effective transfection protocol that allows the introduction of Dicer-substrate interference RNAs (DsiRNAs) for the modulation of gene expression and its characterization during regeneration.
棘皮动物的功能研究已经简化为使用药理学处理。调节再生组织基因表达的能力可以阐明这个过程中的潜在效应物。在这里，我们描述了一种有效的转染方案，允许引入 Dicer 底物干扰 RNA（DsiRNA）来调节基因表达，并在再生过程中对其进行表征。
