Global Centre for Environmental Remediation, The University of Newcastle, Callaghan New South Wales, Australia.
Cooperative Research Centre for Contamination Assessment and Remediation of the Environments, The University of Newcastle, Callaghan, New South Wales, Australia.
Environ Toxicol Chem. 2021 Mar;40(3):792-798. doi: 10.1002/etc.4905. Epub 2020 Dec 10.
Per- and polyfluoroalkyl substances (PFAS) have emerged as contaminants of global concern. Among several PFAS, perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are persistent and bioaccumulative compounds. We investigated the cyto-genotoxic potential of PFOS to Allium cepa root meristem cells. The A. cepa root tips were exposed to 6 different concentrations (1-100 mg L ) of PFOS for 48 h. Reduction in mitotic index and chromosomal aberrations was measured as genotoxic endpoints in meristematic root cells. Exposure to PFOS significantly affected cell division by reducing the miotic index at higher concentrations (>10 mg L ). The median effect concentration of PFOS to elicit cytotoxicity based on the mitotic index was 43.2 mg L . Exposure to PFOS significantly increased chromosomal aberrations at concentrations >25 mg L . The common aberrations were micronuclei, vagrant cells, and multipolar anaphase. The alkaline comet assay revealed a genotoxic potential of PFOS with increased tail DNA percentage at concentrations >25 mg L . To our knowledge, this is the first study to report the cyto-genotoxic potential of PFOS in higher plants. Environ Toxicol Chem 2021;40:792-798. © 2020 SETAC.
全氟和多氟烷基物质（PFAS）已成为全球关注的污染物。在几种 PFAS 中，全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）是持久性和生物累积性化合物。我们研究了 PFOS 对洋葱根尖细胞的细胞遗传毒性。将洋葱根尖暴露于 6 种不同浓度（1-100mg/L）的 PFOS 中 48 小时。有丝分裂指数和染色体畸变作为遗传毒性终点来测量根尖分生细胞中的毒性。在较高浓度（>10mg/L）下，PFOS 显著影响细胞分裂，降低有丝分裂指数。基于有丝分裂指数，PFOS 引起细胞毒性的中效浓度为 43.2mg/L。在浓度>25mg/L 时，PFOS 显著增加染色体畸变。常见的畸变包括微核、游走细胞和多极后期。碱性彗星试验显示，PFOS 具有遗传毒性，在浓度>25mg/L 时，尾部 DNA 百分比增加。据我们所知，这是首次报道 PFOS 对高等植物的细胞遗传毒性。环境毒理化学 2021；40：792-798。2020 年 SETAC 版权所有。
