Department of Chemistry, Faculty of Science, Vali-e-Asr University of Rafsanjan, 77188-97111, Iran.
Department of Chemistry, Faculty of Science, Vali-e-Asr University of Rafsanjan, 77188-97111, Iran.
Int J Biol Macromol. 2021 Jan 1;166:54-60. doi: 10.1016/j.ijbiomac.2020.10.063. Epub 2020 Oct 17.
Despite the use of layered double hydroxides (LDH) in different electrochemical (bio)sensors, the construction of aptasensors using LDH-based surfaces was not reported to the best of our knowledge. This may be due to the lack of a suitable linker to attach aptamers to the LDH-modified surface. LDH-based aptasensors are established here as very sensitive and reliable devices in serum and cerebrospinal fluid (CSF) analysis. 5'-NH DNA aptamer probes were immobilized on the LDH-based surfaces in a vertical conformation without any linker materials. Due to the low electron conductivity of the LDH, carbon nanotubes (CNT) with high electronic conductivity and high surface area were combined with LDH. Thrombin was used as a model protein for aptasensing. The sensor shows a linear range of 0.005-12,000 pmol L and a limit of detection of 0.1 fmol L. Moreover, the aptasensor was used for the sensing of thrombin in CSF and serum samples acquired from both healthy and patients with different disease.
尽管层状双氢氧化物 (LDH) 已被用于不同的电化学（生物）传感器中，但据我们所知，基于 LDH 的表面并未被用于适配体传感器的构建。这可能是由于缺乏合适的连接子将适体连接到 LDH 修饰的表面上。在此，我们建立了基于 LDH 的适配体传感器，该传感器在血清和脑脊液（CSF）分析中具有非常高的灵敏度和可靠性。5'-NH DNA 适体探针在没有任何连接材料的情况下以垂直构象固定在基于 LDH 的表面上。由于 LDH 的电子电导率较低，因此与具有高电子电导率和高表面积的碳纳米管（CNT）相结合。凝血酶被用作适配体传感的模型蛋白。该传感器显示出 0.005-12,000 pmol L 的线性范围和 0.1 fmol L 的检测限。此外，该适配体传感器还用于从健康和患有不同疾病的患者中获取的 CSF 和血清样本中检测凝血酶。
