Zhang Junjie, Lu Nan, Weng Lixing, Feng Zhihao, Tao Jun, Su Xiaodan, Yu Ruifa, Shi Wenhui, Qiu Qiu, Teng Zhaogang, Wang Lianhui
Key Laboratory for Organic Electronics and Information Displays, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210023, PR China.
Department of Radiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, PR China.
J Colloid Interface Sci. 2021 Feb 1;583:714-721. doi: 10.1016/j.jcis.2020.09.060. Epub 2020 Sep 23.
Deformable materials have garnered widespread attention in biomedical applications. Herein, a controllable, general, and simple alkaline etching strategy was used to synthesize deformable hollow mesoporous organosilica nanocapsules (DMONs), in which multiple organic moieties were homogeneously incorporated into the framework. DMONs with double-, triple-, and even quadruple-hybridized frameworks were prepared by the selective introduction of organosilica precursors in accordance with the chemical homology principle through a surfactant-directed sol-gel procedure and a subsequent etching process in alkaline solution. The triple-hybridized DMONs possessed uniform and controllable diameters (100-330 nm), and large hollow cavities (50-270 nm). Liquid cell electron microscopy images demonstrated that the DMONs were deformable in solution. Elemental mapping images suggested that the organic components were homogeneous distribution within the entire DMONs framework. Statistical analysis of cell proliferation assays showed that breast cancer MCF-7 viability exceeded 85% when the cells are incubated with the triple-hybridized DMONs (800 μg mL) for 24 h. Histological assessments of main organs indicated no tissue injury or necrosis after intravenous injection of the DMONs 7 days (5 mg kg body weight). Quantitative analysis indicated that the cellular uptake of the DMONs was 6-fold higher than that of their hard counterparts when the number of nanoparticles added was 1.25 × 10, and similar results were found for 4 T1 cells. Furthermore, doxorubicin (DOX) loaded triple-hybridized DMONs with a loading efficiency of 16.9 wt%, produced a strong killing effect on tumor cells. Overall, DMONs with various incorporated organic functional groups could serve as novel nanoplatforms for drug delivery in biomedical applications.
可变形材料在生物医学应用中受到了广泛关注。在此，我们采用了一种可控、通用且简单的碱性蚀刻策略来合成可变形的中空介孔有机硅纳米胶囊（DMONs），其中多个有机部分均匀地掺入到骨架中。通过表面活性剂导向的溶胶 - 凝胶过程以及随后在碱性溶液中的蚀刻过程，根据化学同源性原理选择性引入有机硅前驱体，制备了具有双杂化、三杂化甚至四杂化骨架的DMONs。三杂化的DMONs具有均匀且可控的直径（100 - 330 nm）以及大的中空腔（50 - 270 nm）。液池电子显微镜图像表明DMONs在溶液中是可变形的。元素映射图像表明有机成分在整个DMONs骨架内均匀分布。细胞增殖试验的统计分析表明，当细胞与三杂化的DMONs（800 μg mL）孵育24小时时，乳腺癌MCF - 7细胞的活力超过85%。主要器官的组织学评估表明，静脉注射DMONs 7天（5 mg kg体重）后没有组织损伤或坏死。定量分析表明，当添加的纳米颗粒数量为1.25×10时，DMONs的细胞摄取量比其硬质对应物高6倍，4T1细胞也得到了类似结果。此外，载有阿霉素（DOX）的三杂化DMONs，负载效率为16.9 wt%，对肿瘤细胞产生了强烈的杀伤作用。总体而言，具有各种掺入有机官能团的DMONs可作为生物医学应用中药物递送的新型纳米平台。
