Kumar Koushi, Kumar Doddi Shanmukha, Arunasree Marasanapalli Kalle, Paik Pradip
School of Engineering Sciences and Technology, University of Hyderabad, India.
Dalton Trans. 2015 Mar 7;44(9):4308-17. doi: 10.1039/c4dt02549k.
Hollow mesoporous-SiO2 nanocapsules have been synthesized at room temperature using unmodified cowpea Mosaic Virus (CPMV) as a template, and without using any catalyst or surfactant during the synthesis. The average size of the capsules synthesized was ∼200-250 nm with a 60-100 nm hollow core. The resulting nanocapsules were characterized using high resolution transmission electron microscopy (HRTEM). The biocompatibility of the hollow mesoporous SiO2 nanocapsules was investigated with an MTT assay using the RAW 264.7 cells, HepG2 cells (human liver carcinoma cells), and Hek293 cells (human embryonic kidney cells). The nanocapsules were loaded with fluorescent molecules (rhodamine 6G), doxorubicin (DOX) – an anticancer drug, and chloroquine diphosphate (CQDP) – an antimalarial drug, and their release was studied using a UV-Vis spectrometer. The development of surfactant free, bio-safe, hollow and mesoporous SiO2 nanocapsules with CPMV provides a route for the synthesis of porous nanocapsules for drug loading and the sustained delivery of drugs. The synthesis method for hollow mesoporous SiO2 nanocapsules using CPMV is novel, straightforward, and further demonstrates that, in general, nanoformulated capsules can be used for various drug-delivery-based therapeutic applications. To check the in vitro efficacy in medical biotechnology, Hek293 and HepG2 cell lines were used to study the cell viability of DOX-loaded hollow silica nanocapsules. The results show that the bio SiO2 nanocapsules synthesized with CPMV present an effective cargo and are suitable for nanoformulating with DOX, with the resultant nanoformulation showing good promise for killing cancer specific cells.
已在室温下以未修饰的豇豆花叶病毒（CPMV）为模板合成了中空介孔二氧化硅纳米胶囊，并且在合成过程中未使用任何催化剂或表面活性剂。合成的胶囊平均尺寸约为200 - 250纳米，具有60 - 100纳米的中空核心。使用高分辨率透射电子显微镜（HRTEM）对所得纳米胶囊进行了表征。使用RAW 264.7细胞、HepG2细胞（人肝癌细胞）和Hek293细胞（人胚肾细胞）通过MTT试验研究了中空介孔二氧化硅纳米胶囊的生物相容性。纳米胶囊装载了荧光分子（罗丹明6G）、阿霉素（DOX）——一种抗癌药物，以及磷酸氯喹（CQDP）——一种抗疟药物，并使用紫外可见光谱仪研究了它们的释放情况。利用CPMV开发无表面活性剂、生物安全、中空且介孔的二氧化硅纳米胶囊为合成用于药物负载和药物持续递送的多孔纳米胶囊提供了一条途径。使用CPMV合成中空介孔二氧化硅纳米胶囊的方法新颖、直接，并且进一步证明，一般而言，纳米配方胶囊可用于各种基于药物递送的治疗应用。为了检查在医学生物技术中的体外功效，使用Hek293和HepG2细胞系研究了装载DOX的中空二氧化硅纳米胶囊的细胞活力。结果表明，用CPMV合成的生物二氧化硅纳米胶囊具有有效的载药量，适合与DOX进行纳米配方，所得的纳米配方在杀死癌症特异性细胞方面显示出良好的前景。
