Zhang LianXiao, Zhong Hua, Zhang Hui, Ding Caifeng
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, 266042, PR China.
Talanta. 2021 Jan 1;221:121554. doi: 10.1016/j.talanta.2020.121554. Epub 2020 Sep 1.
An increased content of reactive oxygen species (ROS) is a primary feature of tumor cells. When the new homeostasis established by cancer cells with a high ROS level is destroyed, this leads to oxidative stress and apoptosis. In this study, a composite nanosystem was designed in which the DNA structure with the functions of miRNA detection and drug delivery is connected to CeO nanoclusters that exhibit enzyme-like activity to enable them to load drugs together. In addition, based on the concept of sequential catalysis, we used CeO to decompose HO into O with low cytotoxicity, which provides raw materials for the photodynamic therapy (PDT) of the Cy5 fluorescent group modified on the DNA. Subsequently, this is transformed into highly cytotoxic free radicals (OH), and we used PDT to further stimulate the therapeutic ability of doxorubicin (DOX) to improve its effectiveness in killing cancer cells. This composite nanosystem can perform fluorescence detection for miRNA-21 in vitro, intracellular fluorescence imaging, and PDT treatment, and can enhance the effect of DOX.
活性氧（ROS）含量增加是肿瘤细胞的一个主要特征。当具有高ROS水平的癌细胞建立的新稳态被破坏时，这会导致氧化应激和细胞凋亡。在本研究中，设计了一种复合纳米系统，其中具有miRNA检测和药物递送功能的DNA结构与表现出类酶活性的CeO纳米团簇相连，使它们能够共同负载药物。此外，基于顺序催化的概念，我们使用CeO将H₂O₂分解为低细胞毒性的O₂，这为DNA上修饰的Cy5荧光基团的光动力疗法（PDT）提供了原料。随后，其转化为高细胞毒性的自由基（·OH），并且我们使用PDT进一步刺激阿霉素（DOX）的治疗能力以提高其杀伤癌细胞的有效性。这种复合纳米系统可以在体外对miRNA-21进行荧光检测、细胞内荧光成像和PDT治疗，并且可以增强DOX的效果。
