β-羟基丁酸和乙酰乙酸对灌注大鼠胰腺胰岛素和胰高血糖素分泌的影响。

Effect of beta-hydroxybutyrate and acetoacetate on insulin and glucagon secretion from perfused rat pancreas.

作者信息

Ikeda T, Yoshida T, Ito Y, Murakami I, Mokuda O, Tominaga M, Mashiba H

出版信息

Arch Biochem Biophys. 1987 Aug 15;257(1):140-3. doi: 10.1016/0003-9861(87)90552-2.

Abstract

To elucidate the physiological significance of ketone bodies on insulin and glucagon secretion, the direct effects of beta-hydroxybutyrate (BOHB) and acetoacetate (AcAc) infusion on insulin and glucagon release from perfused rat pancreas were investigated. The BOHB or AcAc was administered at concentrations of 10, 1, or 0.1 mM for 30 min at 4.0 ml/min. High-concentration infusions of BOHB and AcAc (10 mM) produced significant increases in insulin release in the presence of 4.4 mM glucose, but low-concentration infusions of BOHB and AcAc (1 and 0.1 mM) caused no significant changes in insulin secretion from perfused rat pancreas. BOHB (10, 1, and 0.1 mM) and AcAc (10 and 1 mM) infusion significantly inhibited glucagon secretion from perfused rat pancreas. These results suggest that physiological concentrations of ketone bodies have no direct effect on insulin release but have a direct inhibitory effect on glucagon secretion from perfused rat pancreas.

摘要

为了阐明酮体对胰岛素和胰高血糖素分泌的生理意义，研究了β-羟基丁酸（BOHB）和乙酰乙酸（AcAc）灌注对灌流大鼠胰腺胰岛素和胰高血糖素释放的直接影响。以4.0 ml/min的流速，将浓度为10、1或0.1 mM的BOHB或AcAc给药30分钟。在存在4.4 mM葡萄糖的情况下，高浓度灌注BOHB和AcAc（10 mM）可显著增加胰岛素释放，但低浓度灌注BOHB和AcAc（1和0.1 mM）对灌流大鼠胰腺的胰岛素分泌无显著影响。灌注BOHB（10、1和0.1 mM）和AcAc（10和1 mM）可显著抑制灌流大鼠胰腺的胰高血糖素分泌。这些结果表明，生理浓度的酮体对胰岛素释放无直接影响，但对灌流大鼠胰腺的胰高血糖素分泌有直接抑制作用。