China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University, Beijing 100048, China.
Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, MD 20705, USA.
Nutrients. 2020 Oct 15;12(10):3148. doi: 10.3390/nu12103148.
Enteropathogenic and enterohemorrhagic are important enteric pathogens that induce hemorrhagic colitis or even fatal hemolytic uremic syndrome. Emerging evidence shows that some bio-actives derived from fruits and vegetables may serve as alternatives to antibiotics for overcoming multidrug resistant infections. In this study, the (Cr) infection model was utilized to mimic -induced acute intestinal inflammation, and the effects of a cruciferous vegetable-derived cancer protective compound, indole-3-carbinol (I3C), on the immune responses of Cr-susceptible C3H/HeN mice were investigated. Dietary I3C significantly inhibited the loss of body weight and the increase in spleen size in Cr infected mice. In addition, I3C treatment reduced the inflammatory response to Cr infection by maintaining anti-inflammatory cytokine IL-22 mRNA levels while reducing expression of other pro-inflammatory cytokines including IL17A, IL6, IL1β, TNF-α, and IFN-γ. Moreover, the serum cytokine levels of IL17, TNF-α, IL12p70, and G-CSF also were down-regulated by I3C in Cr-infected mice. Additionally, dietary I3C specifically enhanced the Cr-specific IgG response to Cr infection. In general, dietary I3C reduced the Cr-induced pro-inflammatory response in susceptible C3H/HeN mice and alleviated the physiological changes and tissue damage induced by Cr infection but not Cr colonization.
肠致病性和肠出血性大肠杆菌是重要的肠道病原体，可引起出血性结肠炎甚至致命性溶血性尿毒综合征。新出现的证据表明，一些源自水果和蔬菜的生物活性物质可能可作为抗生素的替代品，用于克服多重耐药性感染。在这项研究中，利用（Cr）感染模型模拟 - 诱导的急性肠道炎症，并研究十字花科蔬菜衍生的癌症保护化合物吲哚-3-甲醇（I3C）对 Cr 易感 C3H/HeN 小鼠免疫反应的影响。饮食 I3C 显著抑制了 Cr 感染小鼠体重减轻和脾脏增大。此外，I3C 通过维持抗炎细胞因子 IL-22 mRNA 水平，同时降低其他促炎细胞因子（包括 IL17A、IL6、IL1β、TNF-α 和 IFN-γ）的表达，减轻了对 Cr 感染的炎症反应。此外，Cr 感染小鼠的血清细胞因子水平如 IL17、TNF-α、IL12p70 和 G-CSF 也被 I3C 下调。此外，饮食 I3C 特异性增强了 Cr 特异性 IgG 对 Cr 感染的反应。总的来说，饮食 I3C 减轻了易感 C3H/HeN 小鼠中 Cr 诱导的促炎反应，并缓解了 Cr 感染而非 Cr 定植引起的生理变化和组织损伤。
