Sánchez-Sánchez José Luis, Calderón-Díez Laura, Herrero-Turrión Javier, Méndez-Sánchez Roberto, Arias-Buría José L, Fernández-de-Las-Peñas César
Department of Physical Therapy, Universidad de Salamanca, 37007 Salamanca, Spain.
Physical Therapy Department, Mutua Accidentes Laborales, FREMAP, 37007 Salamanca, Spain.
J Clin Med. 2020 Oct 15;9(10):3316. doi: 10.3390/jcm9103316.
Percutaneous electrolysis is an emerging intervention proposed for the management of tendinopathies. Tendon pathology is characterized by a significant cell response to injury and gene expression. No study investigating changes in expression of those genes associated with collagen regeneration and remodeling of extracellular matrix has been conducted. The aim of this pilot study was to investigate gene expression changes after the application of percutaneous electrolysis on experimentally induced Achilles tendinopathy with collagenase injection in an animal model. Fifteen Sprague Dawley male rats were randomly divided into three different groups (no treatment vs. percutaneous electrolysis vs. needling). Achilles tendinopathy was experimentally induced with a single bolus of collagenase injection. Interventions consisted of 3 sessions (one per week) of percutaneous electrolysis or just needling. The rats were euthanized, and molecular expression of genes involved in tendon repair and remodeling, e.g., , , , , was examined at 28 days after injury. Histological tissue changes were determined with hematoxylin-eosin and safranin O analyses. The images of hematoxylin-eosin and Safranin O tissue images revealed that collagenase injection induced histological changes compatible with a tendinopathy. No further histological changes were observed after the application of percutaneous electrolysis or needling. A significant increase in molecular expression of genes was observed in Achilles tendons treated with percutaneous electrolysis to a greater extent than after just needling. The expression of genes also increased, but did not reach statistical significance. This animal study demonstrated that percutaneous electrolysis applied on an experimentally induced Achilles tendinopathy model could increase the expression of some genes associated with collagen regeneration and remodeling of extracellular matrix. The observed gene overexpression was higher with percutaneous electrolysis than with just needling.
经皮电解是一种新兴的用于治疗肌腱病的干预方法。肌腱病理特征为细胞对损伤有显著反应以及基因表达。尚未有研究调查与胶原蛋白再生和细胞外基质重塑相关的那些基因的表达变化。本初步研究的目的是在动物模型中，研究经皮电解应用于胶原酶注射诱导的实验性跟腱病后基因表达的变化。15只雄性斯普拉格-道利大鼠被随机分为三组（未治疗组、经皮电解组、针刺组）。通过单次注射胶原酶诱导实验性跟腱病。干预措施包括3次（每周1次）经皮电解或仅针刺。在损伤后28天对大鼠实施安乐死，并检测参与肌腱修复和重塑的基因（如 、 、 、 ）的分子表达。通过苏木精-伊红和番红O分析确定组织学变化。苏木精-伊红和番红O组织图像显示，胶原酶注射诱导了与肌腱病相符的组织学变化。经皮电解或针刺应用后未观察到进一步的组织学变化。与仅针刺后相比，经皮电解治疗的跟腱中 基因的分子表达有显著增加。 基因的表达也有所增加，但未达到统计学显著性。这项动物研究表明，在实验性诱导的跟腱病模型上应用经皮电解可增加一些与胶原蛋白再生和细胞外基质重塑相关的基因的表达。观察到经皮电解后的基因过表达高于仅针刺。
