Imaging Cardiovascular and Lung Macrophages With the Positron Emission Tomography Sensor Cu-Macrin in Mice, Rabbits, and Pigs.

BACKGROUND

Macrophages, innate immune cells that reside in all organs, defend the host against infection and injury. In the heart and vasculature, inflammatory macrophages also enhance tissue damage and propel cardiovascular diseases.

METHODS

We here use in vivo positron emission tomography (PET) imaging, flow cytometry, and confocal microscopy to evaluate quantitative noninvasive assessment of cardiac, arterial, and pulmonary macrophages using the nanotracer Cu-Macrin-a 20-nm spherical dextran nanoparticle assembled from nontoxic polyglucose.

RESULTS

PET imaging using Cu-Macrin faithfully reported accumulation of macrophages in the heart and lung of mice with myocardial infarction, sepsis, or pneumonia. Flow cytometry and confocal microscopy detected the near-infrared fluorescent version of the nanoparticle (Macrin) primarily in tissue macrophages. In 5-day-old mice, Cu-Macrin PET imaging quantified physiologically more numerous cardiac macrophages. Upon intravenous administration of Cu-Macrin in rabbits and pigs, we detected heightened macrophage numbers in the infarcted myocardium, inflamed lung regions, and atherosclerotic plaques using a clinical PET/magnetic resonance imaging scanner. Toxicity studies in rats and human dosimetry estimates suggest that Cu-Macrin is safe for use in humans.

CONCLUSIONS

Taken together, these results indicate Cu-Macrin could serve as a facile PET nanotracer to survey spatiotemporal macrophage dynamics during various physiological and pathological conditions. Cu-Macrin PET imaging could stage inflammatory cardiovascular disease activity, assist disease management, and serve as an imaging biomarker for emerging macrophage-targeted therapeutics.