2016 - 2018年贝宁南部流行的轮状病毒基因型的分子特征
Molecular characteristics of rotavirus genotypes circulating in the south of Benin, 2016-2018.
作者信息
Agbla Jijoho Michel, Esona Mathew D, Agbankpe Alidehou Jerrold, Capo-Chichi Annick, Gautam Rashi, Dougnon Tamegnon Victorien, Razack Osseni, Bowen Michael D, Bankole Honore Sourou
机构信息
Ministry of Public Health, National Health Laboratory, 01 P.O. Box 418, Cotonou, Benin.
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin.
出版信息
BMC Res Notes. 2020 Oct 19;13(1):485. doi: 10.1186/s13104-020-05332-7.
OBJECTIVE
Rotavirus remains the main causative agent of gastroenteritis in young children in countries that have not yet introduced the vaccine. In Benin, rotavirus vaccine was introduced late December 2019 into the EPI. This study aims to provide pre-vaccination era rotavirus genotyping data in Benin. These data can supplement data from the surveillance system of Ministry of Health of Benin which is supported by the World Health Organization (WHO).
RESULTS
Of the 420 diarrheal stool samples, actively collected in southern Benin from July 2016 through November 2018 from children under 5 years old and suffering from gastroenteritis, 167 (39.8%) samples were rotavirus EIA positive. 186 (44.3%) samples contained amplifiable rotavirus RNA detected by qRT-PCR method and were genotyped using one-step RT-PCR multiplex genotyping method. G1P[8] represents the predominant genotype (32%) followed by the G2P[4] (26%), G3P[6] (16%), G12P[8] (13%) and mixed G and P types (1%). Four samples (2%) could not be assigned both G and P type specificity.
目的
在尚未引入疫苗的国家，轮状病毒仍是幼儿肠胃炎的主要病原体。在贝宁，2019年12月底轮状病毒疫苗被纳入扩大免疫规划（EPI）。本研究旨在提供贝宁疫苗接种前时代的轮状病毒基因分型数据。这些数据可补充由世界卫生组织（WHO）支持的贝宁卫生部监测系统的数据。
结果
2016年7月至2018年11月期间，在贝宁南部积极收集了420份5岁以下患肠胃炎儿童的腹泻粪便样本，其中167份（39.8%）样本轮状病毒酶免疫测定（EIA）呈阳性。186份（44.3%）样本通过定量逆转录聚合酶链反应（qRT-PCR）方法检测到含有可扩增的轮状病毒RNA，并使用一步法逆转录聚合酶链反应多重基因分型方法进行基因分型。G1P[8]是主要基因型（32%），其次是G2P[4]（26%）、G3P[6]（16%）、G12P[8]（13%）和G型与P型混合类型（1%）。4份样本（2%）无法确定G型和P型特异性。
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