2010 年至 2013 年科特迪瓦 5 岁以下儿童中流行的轮状病毒的基因特征。
Genotypic characterization of rotavirus in children under 5 years circulating in Côte D'Ivoire from 2010 to 2013.
机构信息
UFR des Sciences Médicales, Département de Microbiologie, Université Félix Houphouët Boigny, Abidjan, Côte d'Ivoire.
Laboratory of Sentinel Site Surveillance of Paediatric Bacterial Meningitis and Rotavirus Diarrhoea CHU Yopougon, Abidjan, Côte d'Ivoire.
出版信息
Virol J. 2018 Apr 27;15(1):78. doi: 10.1186/s12985-018-0973-z.
BACKGROUND
Rotavirus infection is the most common cause of severe gastroenteritis in children under five years of age in both developed and developing countries. The World Health Organisation (WHO) recommends the surveillance of rotavirus strains prior to vaccine introduction in all applicable countries. The objective of this study was to describe the epidemiological characteristics as well as to determine the circulating genotypes of rotaviruses in Côte d'Ivoire prior to vaccine introduction.
METHODS
The study included children under five years of age who met the inclusion criteria after informed consent had been sort from their parents or guardians. Rotavirus VP6 antigens were detected for each stool sample using Enzyme Immunoassay (EIA). Genotyping of positive EIA samples was performed by reverse-transcriptase-PCR (RT-PCR) assays.
RESULTS
A total of 684 children were recruited. Children aged between 6 and 11 months were the most represented with 34%. Rotavirus VP6 antigens were found in 27.1% (186/684) of samples tested. Commonly detected G genotypes included G12 (46.6% (82/176) and G1 (13.1% (23/176) whilst P[8] (49.8% (91/183) was the most predominant P genotype. Rotavirus G12P[8] was the most predominant strain circulating in Côte d'Ivoire within the period of study and constituted 26.6% of all strains detected.
CONCLUSION
The monitoring of circulating strains will help guide decision-makers in the choice of vaccine. Genotypic variability of circulating rotavirus strains over the years implies there is a need for continuous rotavirus strain surveillance even after vaccine introduction.
背景
轮状病毒感染是发达国家和发展中国家 5 岁以下儿童严重胃肠炎的最常见原因。世界卫生组织（WHO）建议所有适用国家在引入疫苗前监测轮状病毒株。本研究的目的是描述科特迪瓦在引入疫苗前轮状病毒的流行病学特征，并确定其流行基因型。
方法
这项研究包括符合纳入标准的 5 岁以下儿童，在获得其父母或监护人同意后，对其进行研究。使用酶联免疫吸附试验（EIA）检测每份粪便样本中的轮状病毒 VP6 抗原。通过逆转录-聚合酶链反应（RT-PCR）检测 EIA 阳性样本的基因分型。
结果
共招募了 684 名儿童。6-11 个月龄的儿童比例最高，为 34%。27.1%（186/684）的检测样本中发现了轮状病毒 VP6 抗原。常见的 G 基因型包括 G12（46.6%（82/176）和 G1（13.1%（23/176），而 P[8]（49.8%（91/183）是最主要的 P 基因型。轮状病毒 G12P[8]是科特迪瓦研究期间流行的主要菌株，占所有检测到菌株的 26.6%。
结论
监测流行株将有助于为决策者在疫苗选择方面提供指导。多年来流行的轮状病毒株的基因型变异表明，即使在引入疫苗后，也需要对轮状病毒株进行持续监测。
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