UBC27-AIRP3 泛素化复合物通过促进拟南芥中 ABI1 的降解来调节 ABA 信号转导。
The UBC27-AIRP3 ubiquitination complex modulates ABA signaling by promoting the degradation of ABI1 in Arabidopsis.
机构信息
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, 100101 Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, 100049 Beijing, China.
出版信息
Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27694-27702. doi: 10.1073/pnas.2007366117. Epub 2020 Oct 19.
Abstract
Abscisic acid (ABA) is the key phytohormone in plant drought tolerance and stress adaptation. The clade A protein phosphatase 2Cs (PP2Cs) like ABI1 (ABA-INSENSITIVE 1) work as coreceptors of ABA and regulate multiple ABA responses. Ubiquitination of ABI1 has been proven to play important regulatory roles in ABA signaling. However, the specific ubiquitin conjugating enzyme (E2) involved is unknown. Here, we report that UBC27 is an active E2 that positively regulates ABA signaling and drought tolerance. UBC27 forms the E2-E3 pair with the drought regulator RING E3 ligase AIRP3. Both UBC27 and AIRP3 interact with ABI1 and affect the ubiquitination and degradation of ABI1. ABA activates the expression of , inhibits the proteasome degradation of UBC27, and enhances the interaction between UBC27 and ABI1 to increase its activity. These findings uncover a regulatory mechanism in ABA signaling and drought response and provide a further understanding of the plant ubiquitination system and ABA signaling pathway.
摘要
脱落酸 (ABA) 是植物耐旱性和应激适应的关键植物激素。类 A 蛋白磷酸酶 2C（PP2C），如 ABI1（ABA 不敏感 1），作为 ABA 的核心受体，调节多种 ABA 反应。ABI1 的泛素化已被证明在 ABA 信号转导中发挥重要的调节作用。然而，具体涉及的泛素连接酶 (E2) 尚不清楚。在这里，我们报告 UBC27 是一种积极的 E2，可正向调节 ABA 信号和耐旱性。UBC27 与干旱调节剂 RING E3 连接酶 AIRP3 形成 E2-E3 对。UBC27 和 AIRP3 均与 ABI1 相互作用，并影响 ABI1 的泛素化和降解。ABA 激活的表达，抑制蛋白酶体降解 UBC27，并增强 UBC27 与 ABI1 的相互作用，以提高其活性。这些发现揭示了 ABA 信号和干旱反应的调控机制，为植物泛素化系统和 ABA 信号通路提供了进一步的认识。
相似文献
1
The UBC27-AIRP3 ubiquitination complex modulates ABA signaling by promoting the degradation of ABI1 in Arabidopsis.UBC27-AIRP3 泛素化复合物通过促进拟南芥中 ABI1 的降解来调节 ABA 信号转导。
Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27694-27702. doi: 10.1073/pnas.2007366117. Epub 2020 Oct 19.
2
Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases.PUB12/13 U-box E3 连接酶对脱落酸共受体ABI1的降解作用。
Nat Commun. 2015 Oct 20;6:8630. doi: 10.1038/ncomms9630.
3
RBR-Type E3 Ligases and the Ubiquitin-Conjugating Enzyme UBC26 Regulate Abscisic Acid Receptor Levels and Signaling.RBR 型 E3 连接酶和泛素结合酶 UBC26 调节脱落酸受体水平和信号转导。
Plant Physiol. 2020 Apr;182(4):1723-1742. doi: 10.1104/pp.19.00898. Epub 2019 Nov 7.
4
New players in ABA signaling: identification of PUB12/13 involved in degradation of ABA co-receptor ABI1.脱落酸信号通路中的新成员：参与脱落酸共受体ABI1降解的PUB12/13的鉴定
Sci China Life Sci. 2015 Nov;58(11):1173-4. doi: 10.1007/s11427-015-4947-8. Epub 2015 Oct 28.
5
Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling.拟南芥 C3HC4-RING 指 E3 泛素连接酶 AtAIRP4 正向调控应激响应的脱落酸信号。
J Integr Plant Biol. 2016 Jan;58(1):67-80. doi: 10.1111/jipb.12364. Epub 2015 Nov 19.
6
The MATH-BTB BPM3 and BPM5 subunits of Cullin3-RING E3 ubiquitin ligases target PP2CA and other clade A PP2Cs for degradation.Cullin3-RING E3 泛素连接酶的 MATH-BTB BPM3 和 BPM5 亚基将 PP2CA 和其他 A 类 PP2C 靶向降解。
Proc Natl Acad Sci U S A. 2019 Jul 30;116(31):15725-15734. doi: 10.1073/pnas.1908677116. Epub 2019 Jul 15.
7
Pepper E3 ligase CaAIRE1 promotes ABA sensitivity and drought tolerance by degradation of protein phosphatase CaAITP1.辣椒 E3 连接酶 CaAIRE1 通过降解蛋白磷酸酶 CaAITP1 促进 ABA 敏感性和耐旱性。
J Exp Bot. 2021 May 28;72(12):4520-4534. doi: 10.1093/jxb/erab138.
8
ABA signaling is negatively regulated by GbWRKY1 through JAZ1 and ABI1 to affect salt and drought tolerance.ABA 信号受 GbWRKY1 通过 JAZ1 和 ABI1 的负调控，影响盐和干旱胁迫耐受性。
Plant Cell Rep. 2020 Feb;39(2):181-194. doi: 10.1007/s00299-019-02480-4. Epub 2019 Nov 12.
9
The Arabidopsis RING E3 ubiquitin ligase AtAIRP3/LOG2 participates in positive regulation of high-salt and drought stress responses.拟南芥 RING E3 泛素连接酶 AtAIRP3/LOG2 参与正向调控高盐和干旱胁迫反应。
Plant Physiol. 2013 Jul;162(3):1733-49. doi: 10.1104/pp.113.220103. Epub 2013 May 21.
10
Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1.通过蛋白磷酸酶2C ABI1和HAB1的联合失活增强拟南芥对脱落酸的敏感性并减少水分消耗
Plant Physiol. 2006 Aug;141(4):1389-99. doi: 10.1104/pp.106.081018. Epub 2006 Jun 23.
引用本文的文献
1
The StUBC18-StPUB40 pair negatively regulate drought stress tolerance and influences tuber yield in potato.StUBC18-StPUB40蛋白对负向调控马铃薯的干旱胁迫耐受性并影响块茎产量。
Hortic Res. 2025 Jun 10;12(9):uhaf145. doi: 10.1093/hr/uhaf145. eCollection 2025 Sep.
2
GhTOPP4aD and GhRAF36 inversely regulate cotton (Gossypium hirsutum) response to ABA and salt stress through reversible phosphorylation of GhABI1.GhTOPP4aD和GhRAF36通过对GhABI1的可逆磷酸化作用，反向调控棉花（陆地棉）对脱落酸和盐胁迫的响应。
Plant Biotechnol J. 2025 Sep;23(9):3561-3580. doi: 10.1111/pbi.70166. Epub 2025 Jun 8.
3
OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling.OsPRMT6b通过对脱落酸信号的反馈抑制来平衡植物生长和高温胁迫。
Nat Commun. 2025 Jun 4;16(1):5173. doi: 10.1038/s41467-025-60350-y.
4
Unveiling the regulatory role of GRP7 in ABA signal-mediated mRNA translation efficiency regulation.揭示GRP7在脱落酸（ABA）信号介导的mRNA翻译效率调控中的调节作用。
Nat Commun. 2025 Apr 26;16(1):3947. doi: 10.1038/s41467-025-59329-6.
5
Genome-wide analysis of CHYR gene family and BnA03.CHYR.1 functional verification under salt stress in Brassica napus L.甘蓝型油菜中CHYR基因家族的全基因组分析及盐胁迫下BnA03.CHYR.1的功能验证
BMC Plant Biol. 2025 Mar 20;25(1):363. doi: 10.1186/s12870-025-06343-x.
6
The Arabidopsis F-box protein FBS associated with the helix-loop-helix transcription factor FAMA involved in stomatal immunity.拟南芥F-box蛋白FBS与参与气孔免疫的螺旋-环-螺旋转录因子FAMA相关。
Plant Mol Biol. 2025 Mar 20;115(2):48. doi: 10.1007/s11103-025-01577-7.
7
Perception of viral infections and initiation of antiviral defence in rice.水稻对病毒感染的感知及抗病毒防御的启动
Nature. 2025 May;641(8061):173-181. doi: 10.1038/s41586-025-08706-8. Epub 2025 Mar 12.
8
Role of Arabidopsis monomeric E3 ubiquitin ligases in the ABA signaling pathway.拟南芥单体E3泛素连接酶在脱落酸信号通路中的作用。
BMB Rep. 2025 Apr;58(4):147-157. doi: 10.5483/BMBRep.2024-0146.
9
OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice.OsPP2C49是脱落酸信号通路中的一个负调控因子，对水稻产量具有正向调控作用。
Rice (N Y). 2024 Oct 23;17(1):65. doi: 10.1186/s12284-024-00746-4.
10
Research Progress in the Regulation of the ABA Signaling Pathway by E3 Ubiquitin Ligases in Plants.植物中 E3 泛素连接酶对 ABA 信号通路的调控研究进展。
Int J Mol Sci. 2024 Jun 28;25(13):7120. doi: 10.3390/ijms25137120.
本文引用的文献
1
COP1 destabilizes DELLA proteins in .在……中，COP1使DELLA蛋白不稳定。 （你提供的原文不完整，这里只能根据现有内容尽量准确翻译）
Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13792-13799. doi: 10.1073/pnas.1907969117. Epub 2020 May 29.
2
Abscisic acid dynamics, signaling, and functions in plants.脱落酸的动态、信号转导及其在植物中的功能。
J Integr Plant Biol. 2020 Jan;62(1):25-54. doi: 10.1111/jipb.12899.
3
RBR-Type E3 Ligases and the Ubiquitin-Conjugating Enzyme UBC26 Regulate Abscisic Acid Receptor Levels and Signaling.RBR 型 E3 连接酶和泛素结合酶 UBC26 调节脱落酸受体水平和信号转导。
Plant Physiol. 2020 Apr;182(4):1723-1742. doi: 10.1104/pp.19.00898. Epub 2019 Nov 7.
4
The MATH-BTB BPM3 and BPM5 subunits of Cullin3-RING E3 ubiquitin ligases target PP2CA and other clade A PP2Cs for degradation.Cullin3-RING E3 泛素连接酶的 MATH-BTB BPM3 和 BPM5 亚基将 PP2CA 和其他 A 类 PP2C 靶向降解。
Proc Natl Acad Sci U S A. 2019 Jul 30;116(31):15725-15734. doi: 10.1073/pnas.1908677116. Epub 2019 Jul 15.
5
Intact leaf gas exchange provides a robust method for measuring the kinetics of stomatal conductance responses to abscisic acid and other small molecules in and grasses.完整叶片气体交换为测量被子植物和禾本科植物气孔导度对脱落酸及其他小分子的响应动力学提供了一种可靠的方法。
Plant Methods. 2019 Apr 17;15:38. doi: 10.1186/s13007-019-0423-y. eCollection 2019.
6
ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA.ABA 抑制豆蔻酰化作用，并诱导 RGLG1 E3 连接酶的易位，从而促进 PP2CA 的核降解。
Plant J. 2019 Jun;98(5):813-825. doi: 10.1111/tpj.14274. Epub 2019 Mar 12.
7
Crystal structure of the Ube2K/E2-25K and K48-linked di-ubiquitin complex provides structural insight into the mechanism of K48-specific ubiquitin chain synthesis.Ube2K/E2-25K 与 K48 连接的二泛素复合物的晶体结构为 K48 特异性泛素链合成机制提供了结构见解。
Biochem Biophys Res Commun. 2018 Nov 17;506(1):102-107. doi: 10.1016/j.bbrc.2018.10.067. Epub 2018 Oct 16.
8
Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response.拟南芥 XIV 组泛素连接酶 AtUBC32、AtUBC33 和 AtUBC34 在干旱胁迫响应中起负调控作用。
J Plant Physiol. 2018 Nov;230:73-79. doi: 10.1016/j.jplph.2018.08.010. Epub 2018 Aug 29.
9
The sHSP22 Heat Shock Protein Requires the ABI1 Protein Phosphatase to Modulate Polar Auxin Transport and Downstream Responses.sHSP22 热休克蛋白需要 ABI1 蛋白磷酸酶来调节极性生长素运输和下游反应。
Plant Physiol. 2018 Mar;176(3):2406-2425. doi: 10.1104/pp.17.01206. Epub 2017 Dec 29.
10
AtRAE1 is involved in degradation of ABA receptor RCAR1 and negatively regulates ABA signalling in Arabidopsis.AtRAE1 参与 ABA 受体 RCAR1 的降解，负调控拟南芥中的 ABA 信号。
Plant Cell Environ. 2018 Jan;41(1):231-244. doi: 10.1111/pce.13086. Epub 2017 Nov 8.
Zotero 插件