Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada.
Cadham Provincial Laboratory, Winnipeg, Canada.
Antimicrob Agents Chemother. 2020 Dec 16;65(1). doi: 10.1128/AAC.00865-20.
Here, we characterize the genes from three clinical isolates recovered from Canadian patients. Each sequence was individually overexpressed in BW25113, and antimicrobial susceptibility testing was performed to assess their role in fosfomycin resistance. The findings from this study identify and functionally characterize FosA3, FosA8, and novel FosA7 members and highlight the importance of phenotypic characterization of genes.
在这里,我们对从加拿大患者身上分离出的三个临床分离株的基因进行了特征描述。每个序列都在 BW25113 中单独过表达,并进行了抗菌药物敏感性测试,以评估它们在磷霉素耐药中的作用。本研究的结果鉴定并功能表征了 FosA3、FosA8 和新型 FosA7 成员,并强调了对基因进行表型特征描述的重要性。