Department of Food Science and Nutrition and Center for Nutrition Research, Institute for Food Safety and Health, Illinois Institute of Technology, Chicago, IL 60616, USA.
Molecules. 2020 Oct 17;25(20):4777. doi: 10.3390/molecules25204777.
Red raspberries (RRB) are high in anthocyanin- and ellagitannin- type (poly)phenols. This study aimed to investigate the effect of 4-week RRB supplementation on (poly)phenolic metabolism in adults with prediabetes and insulin-resistance (PreDM-IR); and whether adding fructo-oligosaccharides (FOS), prebiotics, would augment the microbial metabolites of RRB (poly)phenols. In a randomized crossover clinical trial, subjects (n = 35: PreDM-IR, n = 25; healthy Reference group, n = 10) consumed 1 cup RRB (fresh weight equivalence) per day and RRB with 8 g FOS per day each for 4 weeks in random order separated by 4-week washout. Plasma and urinary (poly)phenolic metabolites were characterized after (0-24h) consuming a RRB-based test drink (2 cups RRB) at baseline/week 0 and again after 4-week supplementations. A total of 123 (poly)phenolic metabolites were quantified. After 4-week RRB supplementation, several metabolite groups were significantly increased ( < 0.05), including urolithins, phenyl-γ-valerolactones, and phenolic acids. Supplementing FOS with RRB for 4 weeks enhanced benzoic acid derivatives compared to the baseline ( < 0.05). Specific effects of supplementation by metabolic status indicated 4-week RRB supplementation significantly increased microbial metabolites that were lower in PreDM-IR group. Our results suggest alterations in the capacity of PreDM-IR group to metabolize and render bioavailable raspberry-derived (poly)phenols when consumed regularly.
红树莓富含花色苷和鞣花单宁型（多）酚类物质。本研究旨在探讨 4 周红树莓补充剂对前驱糖尿病和胰岛素抵抗（PreDM-IR）成年人（多）酚代谢的影响；以及添加低聚果糖（FOS），益生元是否会增强红树莓（多）酚的微生物代谢物。在一项随机交叉临床试验中，受试者（n = 35：PreDM-IR，n = 25；健康参考组，n = 10）每天随机顺序分别摄入 1 杯红树莓（新鲜重量当量）和每天 8 克 FOS 的红树莓，每个干预周期持续 4 周，中间间隔 4 周洗脱期。在基线/第 0 周和 4 周补充后，饮用红树莓基测试饮料（2 杯红树莓）后 0-24 小时，分别对血浆和尿液（多）酚代谢物进行特征分析。共定量了 123 种（多）酚代谢物。在 4 周的红树莓补充后，几个代谢物组显著增加（ < 0.05），包括尿石素、苯-γ-缬草酸内酯和酚酸。与基线相比，4 周补充红树莓和 FOS 显著增加了苯甲酸衍生物（ < 0.05）。按代谢状态补充的特定效果表明，4 周红树莓补充显著增加了在 PreDM-IR 组中含量较低的微生物代谢物。我们的研究结果表明，PreDM-IR 组在经常食用时，代谢和呈现红树莓衍生（多）酚的能力发生了变化。
