Department of Microbiology, Faculty of Science, Ain Shams University, El- Khalyfa El-Mamoun Street, Abbasya, Cairo, Egypt.
Department of Chemistry, Faculty of Science, Port Said University, Port Said, 42521, Egypt.
Sci Rep. 2024 Oct 23;14(1):25020. doi: 10.1038/s41598-024-75147-0.
Using plant extracts in the green synthesis of nanoparticles has become an environmentally acceptable approach. In our study, copper oxide nanoparticles (CuO NPs) were synthesized using ethanolic extracts of Azadirachta indica and Simmondsia chinensis. CuO NP formation was confirmed by the change in color and by UV‒visible spectroscopy (CuO NPs peaked at a wavelength of 344 nm). TEM images confirmed the semispherical shape of the CuO NPs, with particle sizes ranging from 30.9 to 10.7 nm. The antibacterial activity of these NPs was evaluated by using the agar diffusion method against clinical isolates, including methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Pseudomonas aeruginosa, Acinetobacter spp., Klebsiella pneumoniae, and Stenotrophomonas maltophilia. The minimum inhibitory concentration (MIC) of CuO NPs ranged from 62.5 to 125 µg/ml. In contrast, the antioxidant activity and antibiofilm activity of CuO NPs ranged from 31.1 to 92.2% at 125-500 µg/ml and 62.2-95%, respectively, at 125 -62.5 µg/ml. Our results confirmed that CuO NPs had IC50s of 383.41 ± 3.4 and 402.73 ± 1.86 at 250 µg/mL against the HBF4 cell line. Molecular docking studies with CuO NPs suggested that penicillin-binding protein 4 (PBP4) and beta-lactamase proteins (OXA-48) strongly bind to S. aureus and K. pneumoniae, respectively, with CuO NPs. Our study confirms the promising use of CuO NPs in treating pathogenic bacteria and that CuO NPs could be possible alternative antibiotics. This study supports the pharmaceutical and healthcare sectors in Egypt and worldwide.
使用植物提取物进行纳米粒子的绿色合成已经成为一种环境可接受的方法。在我们的研究中，使用印楝和霍霍巴的乙醇提取物合成了氧化铜纳米粒子（CuO NPs）。通过颜色变化和紫外-可见光谱（CuO NPs 在 344nm 处达到峰值）证实了 CuO NP 的形成。TEM 图像证实了 CuO NPs 的半球形形状，粒径范围为 30.9-10.7nm。通过琼脂扩散法评估了这些 NPs 对临床分离株的抗菌活性，包括耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌、铜绿假单胞菌、不动杆菌属、肺炎克雷伯菌和嗜麦芽窄食单胞菌。CuO NPs 的最小抑菌浓度（MIC）范围为 62.5-125μg/ml。相比之下，CuO NPs 的抗氧化和抗生物膜活性在 125-500μg/ml 时范围为 31.1-92.2%，在 125-62.5μg/ml 时范围为 62.2-95%。我们的结果证实，CuO NPs 在 250μg/ml 时对 HBF4 细胞系的 IC50 分别为 383.41±3.4 和 402.73±1.86μg/ml。CuO NPs 的分子对接研究表明，青霉素结合蛋白 4（PBP4）和β-内酰胺酶蛋白（OXA-48）分别与金黄色葡萄球菌和肺炎克雷伯菌强烈结合。我们的研究证实了 CuO NPs 在治疗致病菌方面的应用前景，并且 CuO NPs 可能是潜在的抗生素替代品。本研究支持埃及乃至全球的制药和医疗保健行业。
