Ye Weiwei, Zhang Yu, Hu Wei, Wang Liwen, Zhang Yu, Wang Ping
Key Laboratory of E&M, MOE, Zhejiang University of Technology, Hangzhou 310023, China.
College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou 310023, China.
Nanomaterials (Basel). 2020 Oct 16;10(10):2044. doi: 10.3390/nano10102044.
A sensitive fluorescence resonance energy transfer (FRET) biosensor is proposed to detect 8-hydroxy-2'-deoxyguanosine (8-OHdG), which is a typical DNA oxidation damage product excreted in human urine. The FRET biosensor was based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors. Gold nanoparticles were encapsulated in zeolitic imidazolate framework-8 to form Au@ZIF-8 nanoparticles as signal quenchers. CDs and Au@ZIF-8 nanoparticles were biofunctionalized by 8-OHdG antibody. The capture of 8-OHdG on the membrane substrates can bring Au@ZIF-8 nanoparticles closely to CDs. With 350 nm excitation, the fluorescence of CDs was quenched by Au@ZIF-8 nanoparticles and FRET effect occurred. The quenching efficiency was analyzed. The limit of detection (LOD) was 0.31 nM. Interference experiments of the FRET biosensor showed good specificity for 8-OHdG detection. The biosensor could detect urinary 8-OHdG sensitively and selectively with simple sample pretreatment processes. It shows applicability for detecting biomarkers of DNA damage in urine or other biological fluids.
一种灵敏的荧光共振能量转移（FRET）生物传感器被用于检测8-羟基-2'-脱氧鸟苷（8-OHdG），它是一种典型的DNA氧化损伤产物，可在人体尿液中排出。该FRET生物传感器基于碳点（CDs）修饰的纳米多孔氧化铝膜，其中CDs作为荧光供体。金纳米颗粒被封装在沸石咪唑酯骨架-8中，形成Au@ZIF-8纳米颗粒作为信号猝灭剂。CDs和Au@ZIF-8纳米颗粒通过8-OHdG抗体进行生物功能化。膜基质上8-OHdG的捕获可使Au@ZIF-8纳米颗粒靠近CDs。在350nm激发下，CDs的荧光被Au@ZIF-8纳米颗粒猝灭，发生FRET效应。分析了猝灭效率。检测限（LOD）为0.31 nM。FRET生物传感器的干扰实验表明其对8-OHdG检测具有良好的特异性。该生物传感器通过简单的样品预处理过程，能够灵敏且选择性地检测尿液中的8-OHdG。它显示出在检测尿液或其他生物流体中DNA损伤生物标志物方面的适用性。
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