Discovering the Potent Inhibitors Against and by Repurposing the Natural Product Compounds.

In the present study, we screened 502 natural product compounds against the growth of (.) . Then, the novel and potent identified compounds were further evaluated for their efficacies using viability and cytotoxicity assays. The inhibitory effects of the selected compounds were evaluated using "rodent strain" in mice model. Three potent compounds, namely, Rottlerin (RL), Narasin (NR), Lasalocid acid (LA), exhibited the lowest IC (half-maximal inhibitory concentration) as follows: 5.45 ± 1.20 μM for RL, 1.86 ± 0.66 μM for NR, and 3.56 ± 1.41 μM for LA. The viability result revealed the ability of RL and LA to prevent the regrowth of treated parasite at 4 × IC and 2 × IC, respectively, while 4 × IC of NR was sufficient to stop the regrowth of parasite. The hematology parameters of were different in the NR-treated groups as compared to the infected/untreated group. Interestingly, intraperitoneal administration of NR exhibiting inhibition in the growth of in mice was similar to that observed after administration of the commonly used antibabesial drug, diminazene aceturate (DA) (76.57% for DA, 74.73% for NR). Our findings indicate the richness of natural product compounds by novel potent antibabesial candidates, and the identified potent compounds, especially NR, might be used for the treatment of animal babesiosis.