霍诺酚通过激活 SIRT3 减轻放射性脑损伤。
Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3.
机构信息
Department of Radiation Oncology, Shenzhen People's Hospital, the First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China; Second Clinical Medicine College of Jinan University, Shenzhen, China.
Department of Nephrology, Shenzhen People's Hospital, Second Clinical Medicine College of Jinan University, Shenzhen, China.
出版信息
J Int Med Res. 2020 Oct;48(10):300060520963993. doi: 10.1177/0300060520963993.
OBJECTIVE
Sirtuin 3 (SIRT3) plays a vital role in regulating oxidative stress in tissue injury. The aim of this study was to evaluate the radioprotective effects of honokiol (HKL) in a zebrafish model of radiation-induced brain injury and in HT22 cells.
METHODS
The levels of reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were evaluated in the zebrafish brain and HT22 cells. The expression levels of SIRT3 and cyclooxygenase-2 (COX-2) were measured using western blot assays and real-time polymerase chain reaction (RT-PCR).
RESULTS
HKL treatment attenuated the levels of ROS, TNF-α, and IL-1β in both the and models of irradiation injury. Furthermore, HKL treatment increased the expression of SIRT3 and decreased the expression of COX-2. The radioprotective effects of HKL were achieved via SIRT3 activation.
CONCLUSIONS
HKL attenuated oxidative stress and pro-inflammatory responses in a SIRT3-dependent manner in radiation-induced brain injury.
目的
Sirtuin 3（SIRT3）在调节组织损伤中的氧化应激中起着至关重要的作用。本研究旨在评估和厚朴酚（HKL）在放射诱导脑损伤的斑马鱼模型和 HT22 细胞中的放射保护作用。
方法
评估斑马鱼大脑和 HT22 细胞中的活性氧（ROS）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。使用 Western blot 分析和实时聚合酶链反应（RT-PCR）测量 SIRT3 和环氧化酶-2（COX-2）的表达水平。
结果
HKL 处理减轻了 和 两种照射损伤模型中 ROS、TNF-α 和 IL-1β 的水平。此外，HKL 处理增加了 SIRT3 的表达，降低了 COX-2 的表达。HKL 的放射保护作用是通过 SIRT3 激活实现的。
结论
HKL 通过 SIRT3 依赖性方式减轻了放射诱导脑损伤中的氧化应激和促炎反应。
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