Dessì-Fulgheri P, Cocco F, Glorioso N, Bandiera F, Madeddu P, Rappelli A
Patologia Medica II, University of Sassari, Italy.
Clin Exp Hypertens A. 1987;9(8-9):1383-90. doi: 10.3109/10641968709158991.
The direct measurement of active renin with monoclonal antibodies (IRMA) in plasma from hypertensive patients was compared with the traditional PRA method. Two monoclonal antibodies were used: 3E8 and 4G1. The first was coupled to magnetizable beads and was used to trap both active and inactive renin from plasma. The second antibody, 4G1, was iodinated and used to detect active renin trapped by 3E8. The correlation coefficient between the two methods was very high (r = 0.98, p less than 0.001) in plasma samples whose PRA values were higher than 2 ng/ml/h; in low renin samples (PRA lower than 2 ng/ml/h) no significant correlation was found (r = 0.12 n.s.). When PRA and IRMA were performed before and after trypsin activation of inactive renin, the percentage of inactive over total renin was 86.8% and 84% as calculated with PRA and IRMA respectively. The direct monoclonal antibodies method for measuring active renin can be usefully adopted, in conjunction with the traditional PRA procedure, in studying both clinical and pathophysiological aspects of the renin-angiotensin system.
将高血压患者血浆中用单克隆抗体（免疫放射分析）直接测定活性肾素的方法与传统的血浆肾素活性（PRA）方法进行了比较。使用了两种单克隆抗体：3E8和4G1。第一种与可磁化的珠子偶联，用于从血浆中捕获活性和非活性肾素。第二种抗体4G1用碘标记，用于检测被3E8捕获的活性肾素。在PRA值高于2 ng/ml/h的血浆样本中，两种方法之间的相关系数非常高（r = 0.98，p < 0.001）；在低肾素样本（PRA低于2 ng/ml/h）中未发现显著相关性（r = 0.12，无统计学意义）。当在对非活性肾素进行胰蛋白酶激活前后进行PRA和免疫放射分析时，用PRA和免疫放射分析分别计算，非活性肾素占总肾素的百分比为86.8%和84%。在研究肾素-血管紧张素系统的临床和病理生理方面时，结合传统的PRA程序，直接用单克隆抗体法测定活性肾素是有用的。
