Morganti A, Turolo L, Pulazzini E, Zanchetti A
Istituto di Clinica Medica IV, Università di Milano, Italy.
Clin Exp Hypertens A. 1987;9(8-9):1367-81. doi: 10.3109/10641968709158990.
In human plasma samples we compared the values of renin activity, determined with a conventional enzymatic assay, with those of immunoreactive renin, determined with a new, direct immunoradiometric assay which employs highly specific monoclonal antibodies, and with those of angiotensin II; the comparative measurements of renin were carried out also in trypsin activated samples of nephric and anephric subjects. We found that, overall, there was a close relationship between renin activity and immunoreactive renin; however, this relationship was absent when the statistical analysis was restricted to plasmas with low or very low renin. We also found that, within a rather wide range of values, angiotensin II was more closely correlated with immunoreactive renin than with renin activity. Trypsin activation increased to a similar extent immunoreactive renin and renin activity in plasma of nephric and anephric subjects and, overall, the values of total renin obtained with the two assays were significantly correlated. The results of these comparative determinations indicate that, in general, the measurement of immunoreactive renin represents a valid alternative to that of renin activity and a reliable index of the activity of the renin-angiotensin system. In addition, studies with trypsin activation suggest that even in the anephric state human plasma contains an inactive enzyme convertible into an active form which has immunological properties similar to those of active renin.
在人体血浆样本中，我们将用传统酶法测定的肾素活性值与用一种新的直接免疫放射测定法（该方法采用高特异性单克隆抗体）测定的免疫反应性肾素值以及血管紧张素II的值进行了比较；肾素的对比测量也在肾性和无肾受试者的胰蛋白酶激活样本中进行。我们发现，总体而言，肾素活性与免疫反应性肾素之间存在密切关系；然而，当统计分析仅限于肾素水平低或极低的血浆时，这种关系并不存在。我们还发现，在相当宽的值范围内，血管紧张素II与免疫反应性肾素的相关性比与肾素活性的相关性更密切。胰蛋白酶激活使肾性和无肾受试者血浆中的免疫反应性肾素和肾素活性增加到相似程度，总体而言，两种测定方法得到的总肾素值显著相关。这些对比测定的结果表明，一般来说，免疫反应性肾素的测量是肾素活性测量的有效替代方法，也是肾素-血管紧张素系统活性的可靠指标。此外，胰蛋白酶激活研究表明，即使在无肾状态下，人体血浆中也含有一种可转化为活性形式的无活性酶，其免疫特性与活性肾素相似。
