Simon D, Badouaille G, Pau B, Guyene T T, Corvol P, Ménard J
Centre de Recherches Clin-Midy, Montpellier, France.
Clin Exp Hypertens A. 1987;9(8-9):1333-40. doi: 10.3109/10641968709158987.
Three monoclonal human renin antibodies have been selected to settle two immunoradiometric assays of human plasma renin (IRMA). The first pair of antibodies 3E8-4G1 recognizes exclusively active renin (AR), as demonstrated by the lack of increase either of the number of AR molecules or the renin enzymatic activity, when plasma is set free of inactive renin (IR) by immunoaffinity chromatography with human renin prosegment monoclonal antibody. The second pair of monoclonal antibodies 4E1-3E8 gives results which are very significantly correlated to those obtained with the 3E8-4G1 pair after trypsin activation. The standardization of renin measurements with use of monoclonal antibodies, and the standardization of renin will allow the exchange of comparable informations between the various laboratories involved in the investigation of the renin-angiotensin system in humans.
已筛选出三种单克隆人肾素抗体，用于建立两种人血浆肾素免疫放射测定法（IRMA）。第一对抗体3E8 - 4G1仅识别活性肾素（AR），这一点通过以下实验得以证明：当用人肾素前体片段单克隆抗体通过免疫亲和色谱法去除血浆中的无活性肾素（IR）时，AR分子数量或肾素酶活性均未增加。第二对单克隆抗体4E1 - 3E8的检测结果与经胰蛋白酶激活后用3E8 - 4G1对所获得的结果具有非常显著的相关性。使用单克隆抗体对肾素测量进行标准化，以及对肾素进行标准化，将使参与人类肾素 - 血管紧张素系统研究的各个实验室之间能够交换可比信息。
