Giles C M, Walport M J, David J, Darke C
Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Clin Exp Immunol. 1987 Aug;69(2):368-74.
Strong expression of MHC Class I determinants had been observed on the erythrocytes of three genetically C4 deficient patients who all had SLE. In a study of 35 other SLE patients who were not C4 deficient, 30 showed a marked increase in the expression of MHC Class I on their erythrocytes. There was a correlation between the expression of erythrocyte Class I and disease activity. The polymorphic HLA determinants were detected by haemagglutination with human cytotoxic antisera from untransfused pregnant women. A shared monomorphic epitope of HLA-A, -B and -C, and beta 2-microglobulin were detected by haemagglutination with monoclonal antibodies. A monoclonal antibody for a monomorphic epitope on MHC Class II alpha and beta chains did not react. Erythrocytes from a group of RA patients and a group of normal controls had moderate and low expression respectively. We suggest that MHC Class I may be induced on erythrocytes maturing in a milieu containing mediators derived from activated cells of the immune system. Aberrant tissue expression of MHC antigens may be more widespread than has been previously recognized in diseases mediated by immune mechanisms.
在三名均患有系统性红斑狼疮（SLE）的遗传性C4缺陷患者的红细胞上观察到了MHC I类决定簇的强表达。在另一项针对35名非C4缺陷的SLE患者的研究中，30名患者的红细胞上MHC I类的表达显著增加。红细胞I类的表达与疾病活动度之间存在相关性。通过与未输血孕妇的人细胞毒性抗血清进行血凝反应来检测多态性HLA决定簇。通过与单克隆抗体进行血凝反应来检测HLA-A、-B和-C以及β2-微球蛋白的共享单态表位。一种针对MHC II类α链和β链上单态表位的单克隆抗体没有发生反应。一组类风湿关节炎（RA）患者和一组正常对照的红细胞分别具有中度和低表达。我们认为，MHC I类可能在免疫系统活化细胞衍生的介质环境中成熟的红细胞上被诱导产生。在免疫机制介导的疾病中，MHC抗原的异常组织表达可能比之前所认识到的更为普遍。
