Wongkuna Supapit, Ghimire Sudeep, Chankhamhaengdecha Surang, Janvilisri Tavan, Scaria Joy
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, United States.
PeerJ. 2021 Apr 27;9:e11050. doi: 10.7717/peerj.11050. eCollection 2021.
A Gram-positive, coccobacillus, white raised and circular with an entire edge colony, and obligately anaerobic bacterium, strain SW178 was isolated from the cecum content of feral chickens in Brookings, South Dakota, USA. The most closely related strain based on 16S rRNA gene sequence analysis of strain SW178 was ATCC 27756 ( ATCC 27756) with 96.94% similarity. The genome of strain SW178 is 3.18 Mbp with G+C content of 46.9 mol%. The optimal temperature and pH for growth in modified brain heart infusion (BHI-M) medium were 45 °C and pH 7.5, respectively. The sole carbon sources of the strain were dextrin, L-fucose, D-galacturonic, α-D-glucose, L-rhamnose and D-sorbitol. The primary cellular fatty acids were C, C and C dimethyl acetal (DMA). Based on the genotypic and phenotypic comparison, we proposed that strain SW178 belong to the genus in the family as a novel species, in which the name is proposed. The type strain is SW178 (= DSM 109242 = CCOS 1886).
菌株SW178是从美国南达科他州布鲁金斯市野生鸡的盲肠内容物中分离出的革兰氏阳性、球杆菌、白色凸起且边缘完整的圆形菌落，专性厌氧菌。基于菌株SW178的16S rRNA基因序列分析，与其亲缘关系最近的菌株是ATCC 27756，相似性为96.94%。菌株SW178的基因组为3.18 Mbp，G+C含量为46.9 mol%。在改良脑心浸液（BHI-M）培养基中生长的最适温度和pH分别为45℃和pH 7.5。该菌株的唯一碳源是糊精、L-岩藻糖、D-半乳糖醛酸、α-D-葡萄糖、L-鼠李糖和D-山梨醇。主要细胞脂肪酸为C、C和C二甲基缩醛（DMA）。基于基因型和表型比较，我们提出菌株SW178属于科中的属，作为一个新物种，其中提出了名称。模式菌株为SW178（=DSM 109242 = CCOS 1886）。
