Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
Shandong Mushroom Industiral Technology Innonation Research Institute, Jining 272000, China.
Molecules. 2022 Apr 4;27(7):2334. doi: 10.3390/molecules27072334.
Flavonoids, which are abundant in plants, are recognized for their antioxidant and anticancer roles in clinical applications. However, little is known about the molecular basis of flavonoid biosynthesis in fungi. In this study, we found that inclusion of leachate of Korshinsk peashrub () in the fermentation medium increased the total flavonoid content of the edible fungus by 23.6% relative to that grown in a control medium. Combined transcriptomic and non-targeted metabolomic analysis of the flavonoid biosynthesis pathway in illustrated that there are important metabolites in the phenylpropanoid, coumarin and isoflavonoid biosynthesis pathways. In addition, we found that certain homologous genes encode phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and chalcone isomerase (CHI) in these biosynthesis pathways. These results, in this study, provide a new line for studying the regulation of flavonoid production in edible fungi.
类黄酮在植物中含量丰富，其在临床应用中具有抗氧化和抗癌作用已得到认可。然而，关于真菌中类黄酮生物合成的分子基础知之甚少。在这项研究中，我们发现，在发酵培养基中加入沙棘叶浸提液可使食用真菌的总类黄酮含量相对于对照培养基中生长的真菌增加 23.6%。对 中类黄酮生物合成途径的转录组和非靶向代谢组学分析表明，在苯丙烷、香豆素和异黄酮生物合成途径中有重要代谢物。此外，我们发现这些生物合成途径中的某些同源基因编码苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）和查尔酮异构酶（CHI）。本研究结果为研究食用真菌中类黄酮的生产调控提供了新途径。
