Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222, Bialystok, Poland.
Dermatological Specialized Center "DERMAL" NZOZ in Bialystok, Poland.
J Pharm Biomed Anal. 2021 Jan 5;192:113656. doi: 10.1016/j.jpba.2020.113656. Epub 2020 Oct 3.
UV phototherapy used in chronic skin diseases causes redox imbalance and pro-inflammatory reactions, especially in the case of unchanged skin cells. To prevent the harmful effects of UV radiation, cannabidiol (CBD) has been used, which has antioxidant and anti-inflammatory properties. Therefore, the aim of this study was to evaluate the effect of CBD on the metabolism of skin keratinocytes in nude rats exposed to UVA/UVB radiation using a proteomic approach. The results obtained with SDS-PAGE/nanoHPLC/QexactiveOrbiTrap show that exposure of rat's skin to UVA/UVB radiation, as well as the action of CBD, significantly modified the expression of proteins involved in inflammation, redox balance and apoptosis. UVA/UVB radiation significantly increased the expression and biological effectiveness of the nuclear factor associated with erythroid factor 2 (Nrf2) and cytoprotective proteins being products of its transcriptional activity, including superoxide dismutase (Cu,Zn-SOD) and the inflammatory response (nuclear receptor coactivator-3 and paralemmin-3), while CBD treatment counteracted and partially eliminated these changes. Moreover, cannabidiol reversed changes in the UV-induced apoptotic pathways by modifying anti-apoptotic and pro-apoptotic factors (apoptosis regulator Bcl-2 and transforming growth factor-β). The results show that CBD maintains keratinocyte proteostasis and therefore could be suggested as a protective measure in the prevention of UV-induced metabolic changes in epidermal keratinocytes.
UV 光疗在慢性皮肤病中的应用会导致氧化还原失衡和促炎反应，尤其是在皮肤细胞不变的情况下。为了防止 UV 辐射的有害影响，已经使用了具有抗氧化和抗炎特性的大麻二酚 (CBD)。因此，本研究的目的是使用蛋白质组学方法评估 CBD 对 UVA/UVB 辐射暴露的无毛大鼠皮肤角质形成细胞代谢的影响。SDS-PAGE/nanoHPLC/QexactiveOrbiTrap 获得的结果表明，大鼠皮肤暴露于 UVA/UVB 辐射以及 CBD 的作用显著改变了参与炎症、氧化还原平衡和细胞凋亡的蛋白质的表达。UVA/UVB 辐射显著增加了与红细胞因子 2 相关的核因子 (Nrf2) 的表达和生物功效，以及其转录活性的产物，包括超氧化物歧化酶 (Cu，Zn-SOD) 和炎症反应 (核受体共激活因子-3 和 paralemmin-3)，而 CBD 处理则对抗并部分消除了这些变化。此外，大麻二酚通过改变抗凋亡和促凋亡因子 (凋亡调节因子 Bcl-2 和转化生长因子-β) 逆转了 UV 诱导的凋亡途径的变化。结果表明，CBD 维持角质形成细胞的蛋白质稳态，因此可以作为预防表皮角质形成细胞中 UV 诱导的代谢变化的保护措施。
