• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大麻二酚对 3D 培养皮肤角质形成细胞中 UV 诱导的细胞内信号变化的影响。

The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes.

机构信息

Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland.

Department of Biochemistry and Molecular Biology, University of Southern Danmark, Campusvej 55, DK-5230 Odense M, Denmark.

出版信息

Int J Mol Sci. 2021 Feb 2;22(3):1501. doi: 10.3390/ijms22031501.

DOI:10.3390/ijms22031501
PMID:33540902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7867360/
Abstract

Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.

摘要

人类表皮角质细胞不断受到紫外线辐射的影响。因此，需要安全有效的化合物来保护皮肤细胞免受这种环境损害。本研究旨在分析植物大麻素-大麻素（CBD）对 UVA/B 照射角质细胞蛋白质组的影响。角质细胞在三维（3D）系统中培养，该系统设计用于紧密模拟表皮条件。研究结果表明，CBD 可预防 UVA/B 辐射的有害影响。CBD 降低了促炎蛋白的表达，包括 TNFα/NFκB 和 IκBKB 复合物，并降低了新合成蛋白质的表达，这些蛋白在 UVA/B 照射的细胞中增加。此外，CBD 增强了 UV 诱导的 20S 蛋白酶体亚基的表达。CBD 还能保护蛋白质结构免受 UV 辐射诱导的 4-羟基壬烯醛（HNE）结合，HNE 主要影响抗氧化酶。CBD 通过其抗氧化/抗炎活性以及对蛋白质生物合成和降解的调节，保护皮肤细胞免受 UVA/B 诱导的变化。将来，应研究其在表皮细胞中的长期使用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/72a25701178b/ijms-22-01501-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/99ed4537fe19/ijms-22-01501-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/5320bf327033/ijms-22-01501-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/e9d89cd6bd3c/ijms-22-01501-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/9bcf125365fe/ijms-22-01501-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/dd7076796e28/ijms-22-01501-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/b24db8de189d/ijms-22-01501-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/dc31c5abc874/ijms-22-01501-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/c62ade473c93/ijms-22-01501-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/72a25701178b/ijms-22-01501-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/99ed4537fe19/ijms-22-01501-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/5320bf327033/ijms-22-01501-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/e9d89cd6bd3c/ijms-22-01501-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/9bcf125365fe/ijms-22-01501-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/dd7076796e28/ijms-22-01501-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/b24db8de189d/ijms-22-01501-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/dc31c5abc874/ijms-22-01501-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/c62ade473c93/ijms-22-01501-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28d6/7867360/72a25701178b/ijms-22-01501-g009.jpg

相似文献

1
The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes.大麻二酚对 3D 培养皮肤角质形成细胞中 UV 诱导的细胞内信号变化的影响。
Int J Mol Sci. 2021 Feb 2;22(3):1501. doi: 10.3390/ijms22031501.
2
Cannabidiol Effects on Phospholipid Metabolism in Keratinocytes from Patients with Psoriasis Vulgaris.大麻二酚对寻常型银屑病患者角质形成细胞磷脂代谢的影响。
Biomolecules. 2020 Feb 28;10(3):367. doi: 10.3390/biom10030367.
3
UV induced changes in proteome of rats plasma are reversed by dermally applied cannabidiol.经皮给予大麻二酚可逆转紫外线诱导的大鼠血浆蛋白质组变化。
Sci Rep. 2021 Oct 19;11(1):20666. doi: 10.1038/s41598-021-00134-8.
4
Cannabidiol Regulates the Expression of Keratinocyte Proteins Involved in the Inflammation Process through Transcriptional Regulation.大麻二酚通过转录调控调节角质形成细胞中参与炎症过程的蛋白表达。
Cells. 2019 Aug 3;8(8):827. doi: 10.3390/cells8080827.
5
Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation.芦丁和抗坏血酸对 UVA 和 UVB 辐射暴露的人皮肤角质形成细胞和成纤维细胞的抗氧化和抗凋亡作用的协同作用。
Arch Dermatol Res. 2019 Apr;311(3):203-219. doi: 10.1007/s00403-019-01898-w. Epub 2019 Feb 19.
6
Modulation of Redox and Inflammatory Signaling in Human Skin Cells Using Phytocannabinoids Applied after UVA Irradiation: In Vitro Studies.使用 UVA 照射后应用的植物大麻素调节人皮肤细胞中的氧化还原和炎症信号：体外研究。
Cells. 2024 Jun 3;13(11):965. doi: 10.3390/cells13110965.
7
The Differences in the Proteome Profile of Cannabidiol-Treated Skin Fibroblasts following UVA or UVB Irradiation in 2D and 3D Cell Cultures.经大麻二酚处理的皮肤成纤维细胞在二维和三维细胞培养中经 UVA 或 UVB 辐射后的蛋白质组特征差异。
Cells. 2019 Aug 28;8(9):995. doi: 10.3390/cells8090995.
8
Therapeutic application of cannabidiol on UVA and UVB irradiated rat skin. A proteomic study.大麻二酚对 UVA 和 UVB 照射大鼠皮肤的治疗应用。一项蛋白质组学研究。
J Pharm Biomed Anal. 2021 Jan 5;192:113656. doi: 10.1016/j.jpba.2020.113656. Epub 2020 Oct 3.
9
Disease-Dependent Antiapoptotic Effects of Cannabidiol for Keratinocytes Observed upon UV Irradiation.UV 照射下观察到大麻二酚对角质形成细胞的疾病相关抗凋亡作用。
Int J Mol Sci. 2021 Sep 15;22(18):9956. doi: 10.3390/ijms22189956.
10
Photo-oxidation products of skin surface squalene mediate metabolic and inflammatory responses to solar UV in human keratinocytes.皮肤表面角鲨烯的光氧化产物介导人类角质形成细胞对太阳紫外线的代谢和炎症反应。
PLoS One. 2012;7(8):e44472. doi: 10.1371/journal.pone.0044472. Epub 2012 Aug 30.

引用本文的文献

1
Therapeutic potential of cannabinoids for treating atopic dermatitis.大麻素治疗特应性皮炎的潜在疗效。
J Cannabis Res. 2025 Aug 16;7(1):57. doi: 10.1186/s42238-025-00317-4.
2
Dietary Principles, Interventions and Oxidative Stress in Psoriasis Management: Current and Future Perspectives.银屑病管理中的饮食原则、干预措施与氧化应激：现状与未来展望
Medicina (Kaunas). 2025 Jul 18;61(7):1296. doi: 10.3390/medicina61071296.
3
Oxidative Stress and Skin Diseases: The Role of Lipid Peroxidation.氧化应激与皮肤疾病：脂质过氧化的作用

本文引用的文献

1
Neuroprotective Effect of Cannabidiol Against Hydrogen Peroxide in Hippocampal Neuron Culture.大麻二酚对过氧化氢诱导的海马神经元培养中的神经保护作用。
Cannabis Cannabinoid Res. 2021 Feb 12;6(1):40-47. doi: 10.1089/can.2019.0102. eCollection 2021.
2
Cannabidiol protects keratinocyte cell membranes following exposure to UVB and hydrogen peroxide.大麻二酚可保护角质形成细胞膜免受 UVB 和过氧化氢的损伤。
Redox Biol. 2020 Sep;36:101613. doi: 10.1016/j.redox.2020.101613. Epub 2020 Jun 23.
3
Cannabidiol (CBD): Perspectives from Pinterest.
Antioxidants (Basel). 2025 May 7;14(5):555. doi: 10.3390/antiox14050555.
4
Stabilization of β-Carotene Liposomes with Chitosan-Lactoferrin Coating System: Vesicle Properties and Anti-Inflammatory In Vitro Studies.壳聚糖-乳铁蛋白包衣系统对β-胡萝卜素脂质体的稳定作用：囊泡性质及体外抗炎研究
Foods. 2025 Mar 12;14(6):968. doi: 10.3390/foods14060968.
5
Discovering the Potential of Cannabidiol for Cosmeceutical Development at the Cellular Level.在细胞水平上探索大麻二酚用于药妆品开发的潜力。
Pharmaceuticals (Basel). 2025 Feb 2;18(2):202. doi: 10.3390/ph18020202.
6
Full-spectrum cannabidiol reduces UVB damage through the inhibition of TGF-β1 and the NLRP3 inflammasome.全谱大麻二酚通过抑制转化生长因子-β1和NLRP3炎性小体减少紫外线B损伤。
Photochem Photobiol. 2025 Jan-Feb;101(1):83-105. doi: 10.1111/php.13993. Epub 2024 Jul 3.
7
Differences in the phospholipid profile of melanocytes and melanoma cells irradiated with UVA and treated with cannabigerol and cannabidiol.UVA 辐射和大麻萜酚与大麻二酚处理后的黑素细胞和黑素瘤细胞中磷脂谱的差异。
Sci Rep. 2023 Sep 26;13(1):16121. doi: 10.1038/s41598-023-43363-9.
8
Therapeutic Potential of Minor Cannabinoids in Dermatological Diseases-A Synthetic Review.治疗皮肤疾病中小分子大麻素的潜力——综述
Molecules. 2023 Aug 20;28(16):6149. doi: 10.3390/molecules28166149.
9
Pleiotropic loci for cannabis use disorder severity in multi-ancestry high-risk populations.多血统高危人群中与大麻使用障碍严重程度相关的多效性位点。
Mol Cell Neurosci. 2023 Jun;125:103852. doi: 10.1016/j.mcn.2023.103852. Epub 2023 Apr 14.
10
Photoprotective Effects of Cannabidiol against Ultraviolet-B-Induced DNA Damage and Autophagy in Human Keratinocyte Cells and Mouse Skin Tissue.大麻二酚对人角质形成细胞和小鼠皮肤组织中紫外线 B 诱导的 DNA 损伤和自噬的光保护作用。
Molecules. 2022 Oct 10;27(19):6740. doi: 10.3390/molecules27196740.
大麻二酚（CBD）：来自Pinterest的观点。
Subst Use Misuse. 2020;55(13):2213-2220. doi: 10.1080/10826084.2020.1797808. Epub 2020 Jul 25.
4
Antioxidant and Neuroprotective Effects Induced by Cannabidiol and Cannabigerol in Rat CTX-TNA2 Astrocytes and Isolated Cortexes.大麻二酚和大麻萜酚对 CTX-TNA2 星形胶质细胞和离体皮质诱导的抗氧化和神经保护作用。
Int J Mol Sci. 2020 May 18;21(10):3575. doi: 10.3390/ijms21103575.
5
Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System.抗坏血酸和芦丁对三维培养系统中皮肤成纤维细胞蛋白质组氧化变化的细胞保护作用。
Nutrients. 2020 Apr 13;12(4):1074. doi: 10.3390/nu12041074.
6
Cannabidiol Effects on Phospholipid Metabolism in Keratinocytes from Patients with Psoriasis Vulgaris.大麻二酚对寻常型银屑病患者角质形成细胞磷脂代谢的影响。
Biomolecules. 2020 Feb 28;10(3):367. doi: 10.3390/biom10030367.
7
Antioxidative and Anti-Inflammatory Properties of Cannabidiol.大麻二酚的抗氧化和抗炎特性
Antioxidants (Basel). 2019 Dec 25;9(1):21. doi: 10.3390/antiox9010021.
8
Using MetaboAnalyst 4.0 for Comprehensive and Integrative Metabolomics Data Analysis.使用MetaboAnalyst 4.0进行全面综合的代谢组学数据分析。
Curr Protoc Bioinformatics. 2019 Dec;68(1):e86. doi: 10.1002/cpbi.86.
9
Cannabidiol promotes apoptosis via regulation of XIAP/Smac in gastric cancer.大麻二酚通过调节 XIAP/Smac 促进胃癌细胞凋亡。
Cell Death Dis. 2019 Nov 7;10(11):846. doi: 10.1038/s41419-019-2001-7.
10
Synergistic Cytoprotective Effects of Rutin and Ascorbic Acid on the Proteomic Profile of 3D-Cultured Keratinocytes Exposed to UVA or UVB Radiation.芦丁和抗坏血酸对 UVA 或 UVB 辐射暴露的 3D 培养角质形成细胞蛋白质组谱的协同细胞保护作用。
Nutrients. 2019 Nov 5;11(11):2672. doi: 10.3390/nu11112672.