Zhu Hong, Zou Xia, Lin ShiXin, Hu Xin, Gao Jun
Department of Gynecology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
Department of Gynecology, The People's Hospital of Jiangxi Province, Nanchang, Jiangxi Province, China.
J Int Med Res. 2020 Oct;48(10):300060519887869. doi: 10.1177/0300060519887869.
Ovarian cancer is one of three malignant tumors of the female reproductive system. Our previous studies showed that the traditional Chinese medicine naringin significantly inhibited the proliferation of platinum-resistant ovarian cancer cells , and that the mechanism may be related to the NF-κB pathway.
The MTT assay was used to detect the sensitivity of SKOV3 and SKOV3/CDDP cells to cisplatin, the effect of different naringin concentrations on the proliferation of SKOV3/CDDP cells, and the reversal of cisplatin resistance in naringin-treated SKOV3/CDDP cells. Western blotting was used to detect β-catenin, c-Myc, and cyclin D1 protein levels in the different cell lines.
MTT results showed that different concentrations of naringin inhibited the proliferation of SKOV3 and SKOV3/CDDP cells, and that the inhibition increased with increasing concentrations and prolonged incubation times. Western blotting revealed that compared with controls (SKOV3/CDDP-0), β-catenin, c-Myc and cyclin D1 proteins levels were significantly decreased in SKOV3/CDDP-C, SKOV3/CDDP-N 20, and SKOV3/CDDP-CN 20 cells, suggesting that naringin inhibited the proliferation of SKOV3/CDDP cells in a concentration and time dependent manner.
Non-cytotoxic naringin reduced the expression of β-catenin, c-Myc, and cyclin D1 in SKOV3/CDDP cells and partially reversed cisplatin resistance in SKOV3/CDDP CN 20 cells.
卵巢癌是女性生殖系统三大恶性肿瘤之一。我们之前的研究表明，中药柚皮苷能显著抑制铂耐药卵巢癌细胞的增殖，其机制可能与NF-κB通路有关。
采用MTT法检测SKOV3和SKOV3/CDDP细胞对顺铂的敏感性、不同浓度柚皮苷对SKOV3/CDDP细胞增殖的影响以及柚皮苷处理的SKOV3/CDDP细胞中顺铂耐药性的逆转情况。采用蛋白质免疫印迹法检测不同细胞系中β-连环蛋白、c-Myc和细胞周期蛋白D1的蛋白水平。
MTT结果显示，不同浓度的柚皮苷均能抑制SKOV3和SKOV3/CDDP细胞的增殖，且抑制作用随浓度增加和孵育时间延长而增强。蛋白质免疫印迹法显示，与对照组（SKOV3/CDDP-0）相比，SKOV3/CDDP-C、SKOV3/CDDP-N 20和SKOV3/CDDP-CN 20细胞中β-连环蛋白、c-Myc和细胞周期蛋白D1的蛋白水平显著降低，表明柚皮苷以浓度和时间依赖性方式抑制SKOV3/CDDP细胞的增殖。
无细胞毒性的柚皮苷降低了SKOV3/CDDP细胞中β-连环蛋白、c-Myc和细胞周期蛋白D1的表达，并部分逆转了SKOV3/CDDP CN 20细胞中的顺铂耐药性。
