DNA 甲基化对甜菜细胞质雄性不育的影响。

The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet.

机构信息

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China.

出版信息

Int J Mol Sci. 2024 Jan 17;25(2):1118. doi: 10.3390/ijms25021118.

DOI:10.3390/ijms25021118

PMID:38256191

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10817047/

Abstract

DNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verified by bisulfite transformation, indicating that the results of WGBS technology were reliable. In addition, 12 differential methylation genes (DMGs) were identified, which were related to carbohydrate and energy metabolism, pollen wall development, and endogenous hormone regulation. Quantitative real-time PCR (qRT-PCR) showed that 75% of DMG expression levels showed negative feedback with methylation level, indicating that DNA methylation can affect gene expression to a certain extent. In addition, we found hypermethylation inhibited gene expression, which laid a foundation for further study on the molecular mechanism of DNA methylation at the epigenetic level in sugar beet male sterility.

摘要

DNA 甲基化广泛存在于高等植物中，能够通过不改变 DNA 序列的调节来控制基因表达。在本研究中，利用 WGBS（全基因组亚硫酸氢盐测序）技术构建了甜菜的全基因组甲基化图谱，并通过亚硫酸氢盐转化验证了 WGBS 的结果，表明 WGBS 技术的结果是可靠的。此外，鉴定到了 12 个差异甲基化基因（DMGs），这些基因与碳水化合物和能量代谢、花粉壁发育和内源激素调节有关。实时定量 PCR（qRT-PCR）显示，75%的 DMG 表达水平与甲基化水平呈负反馈关系，表明 DNA 甲基化可以在一定程度上影响基因表达。此外，我们发现超甲基化抑制了基因表达，这为进一步研究甜菜雄性不育的表观遗传水平上的 DNA 甲基化分子机制奠定了基础。