Ye Jiali, Duan Yang, Hu Gan, Geng Xingxia, Zhang Gaoming, Yan Pengjiao, Liu Zihan, Zhang Lingli, Song Xiyue
College of Agronomy, Northwest A&F UniversityYangling, China.
Front Plant Sci. 2017 Apr 6;8:449. doi: 10.3389/fpls.2017.00449. eCollection 2017.
The thermo-sensitive cytoplasmic male sterility (K-TCMS) system may facilitate hybrid wheat ( L.) seed multiplication and production. The K-TCMS line is completely male sterile during the normal wheat-growing season, whereas its fertility can be restored in a high-temperature environment. To elucidate the molecular mechanisms responsible for male sterility/fertility conversion and candidate genes involved with pollen development in K-TCMS, we employed RNA-seq to sequence the transcriptomes of anthers from K-TCMS line KTM3315A during development under sterile and fertile conditions. We identified 16840 differentially expressed genes (DEGs) in different stages including15157 known genes (15135 nuclear genes and 22 plasmagenes) and 1683 novel genes. Bioinformatics analysis identified possible metabolic pathways involved with fertility based on KEGG pathway enrichment of the DEGs expressed in fertile and sterile plants. We found that most of the genes encoding key enzyme in the phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were significant upregulated in uninucleate, binuclate or trinucleate stage, which both interact with MYB transcription factors, and that link between all play essential roles in fertility conversion. The relevant DEGs were verified by quantitative RT-PCR. Thus, we suggested that phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were involved in fertility conversion of K-TCMS wheat. This will provide a new perspective and an effective foundation for the research of molecular mechanisms of fertility conversion of CMS wheat. Fertility conversion mechanism in thermo-sensitive cytoplasmic male sterile/fertile wheat involves the phenylpropanoid biosynthesis pathway, jasmonate biosynthesis pathway, and MYB transcription factors.
温敏细胞质雄性不育（K-TCMS）系统可能有助于杂交小麦种子的繁殖和生产。K-TCMS系在正常小麦生长季节完全雄性不育，而在高温环境下其育性可以恢复。为了阐明K-TCMS中雄性不育/育性转换的分子机制以及与花粉发育相关的候选基因，我们利用RNA测序对K-TCMS系KTM3315A在不育和可育条件下发育过程中的花药转录组进行了测序。我们在不同阶段鉴定出16840个差异表达基因（DEG），包括15157个已知基因（15135个核基因和22个质体基因）和1683个新基因。基于可育和不育植株中表达的DEG的KEGG通路富集，生物信息学分析确定了与育性相关的可能代谢途径。我们发现，苯丙烷生物合成和茉莉酸生物合成途径中编码关键酶的大多数基因在单核、双核或三核阶段显著上调，这两条途径都与MYB转录因子相互作用，并且它们之间的联系在育性转换中都起着重要作用。相关的DEG通过定量RT-PCR进行了验证。因此，我们认为苯丙烷生物合成和茉莉酸生物合成途径参与了K-TCMS小麦的育性转换。这将为CMS小麦育性转换分子机制的研究提供新的视角和有效的基础。温敏细胞质雄性不育/可育小麦的育性转换机制涉及苯丙烷生物合成途径、茉莉酸生物合成途径和MYB转录因子。
