Venn A J, Dresser D W
National Institute for Medical Research, London, U.K.
J Immunol Methods. 1987 Sep 24;102(2):195-204. doi: 10.1016/0022-1759(87)90077-9.
An attempt has been made to develop a hemolytic plaque assay capable of detecting homophile IgG rheumatoid factor (RF)-producing cells. Anti-immunoglobulin allotype-developing reagents were used to distinguish between target and effector IgG. The hemolytic assay has been used to demonstrate an apparently high level of homophile IgM and IgG RF-producing cells in the spleens and lymph nodes of mice stimulated by LPS. However, it appears that a large proportion of the plaques obtained in these assays are due to an artefact resulting from cross-linking of target and effector molecules by the developing reagents. In the case of IgM RF the artefact depends on the presence of a small contamination of the target IgG by IgM, allowing cross-linking of target and effector IgM by the anti-mu-specific developing reagent. With the IgG RF, cross-reactivity of the rabbit anti-Ighb allotype-developing serum for the 'wrong' (Igha) allotype, normally undetectable, becomes sufficient to be biologically relevant when the developing antibody is complexed by being bound to its target (Ighb) allotype. Nevertheless anti-allotype reagents may afford an accurate means of detecting homophile IgG RF producing cells using other assay systems.
人们试图开发一种能够检测产生嗜同种IgG类风湿因子（RF）细胞的溶血空斑试验。使用抗免疫球蛋白同种异型产生试剂来区分靶标IgG和效应IgG。溶血试验已被用于证明在经脂多糖刺激的小鼠脾脏和淋巴结中，产生嗜同种IgM和IgG RF的细胞水平明显较高。然而，在这些试验中获得的很大一部分空斑似乎是由显色试剂使靶标分子和效应分子交联导致的假象。对于IgM RF，这种假象取决于靶标IgG被IgM轻微污染的存在，使得抗μ特异性显色试剂能够使靶标IgM和效应IgM交联。对于IgG RF，兔抗-Ighb同种异型产生血清对“错误”（Igha）同种异型的交叉反应性，通常无法检测到，但当显色抗体通过与靶标（Ighb）同种异型结合而形成复合物时，就足以产生生物学相关性。尽管如此，抗同种异型试剂可能为使用其他检测系统检测产生嗜同种IgG RF的细胞提供一种准确的方法。
