Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, Piastów Avenue 42, 71-065 Szczecin, Poland.
Chair of Building Materials and Construction Chemistry, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
Int J Mol Sci. 2020 Oct 20;21(20):7748. doi: 10.3390/ijms21207748.
is a bacterium of high clinical and biotechnological importance thanks to its high adaptability to environmental conditions. The increasing incidence of antibiotic-resistant strains has created a need for alternative methods to increase the chance of recovery in infected patients. Various nanomaterials have the potential to be used for this purpose. Therefore, we aimed to study the physiological response of PAO1 to titanium dioxide/silica nanotubes. The results suggest that UV light-irradiated nanomaterial triggers strong agglomeration in the studied bacteria that was confirmed by microscopy, spectrophotometry, and flow cytometry. The effect was diminished when the nanomaterial was applied without initial irradiation, with UV light indicating that the creation of reactive oxygen species could play a role in this phenomenon. The nanocomposite also affected biofilm formation ability. Even though the biomass of biofilms was comparable, the viability of cells in biofilms was upregulated in 48-hour biofilms. Furthermore, from six selected genes, the coding efflux pump was upregulated, which could be associated with an interaction with TiO. The results show that titanium dioxide/silica nanotubes may alter the physiological and metabolic functions of PAO1.
是一种具有高度临床和生物技术重要性的细菌，因为它对环境条件具有很高的适应性。抗生素耐药菌株的发病率不断上升，这就需要寻找替代方法来提高感染患者的康复机会。各种纳米材料具有用于此目的的潜力。因此，我们旨在研究 PAO1 对二氧化钛/硅纳米管的生理反应。结果表明，受紫外线照射的纳米材料会在研究的细菌中引发强烈的聚集，这一点通过显微镜、分光光度法和流式细胞术得到了证实。当纳米材料未经初始照射而应用时，效果会减弱，这表明活性氧的产生可能在这一现象中发挥作用。纳米复合材料还影响生物膜形成能力。尽管生物膜的生物量相当，但 48 小时生物膜中生物膜内细胞的活力上调。此外，在 6 个选定的基因中，编码外排泵的基因上调，这可能与 TiO 的相互作用有关。结果表明，二氧化钛/硅纳米管可能会改变 PAO1 的生理和代谢功能。
