Fenlon S, Ellis I O, Bell J, Todd J H, Elston C W, Blamey R W
Department of Histopathology, Queen's Medical Centre, City Hospital, Nottingham.
J Pathol. 1987 Jul;152(3):169-76. doi: 10.1002/path.1711520305.
Formalin fixed, paraffin embedded tissue from 100 consecutive cases of breast carcinoma were studied for binding with Helix pomatia (HPA) and Ulex Europeus (UEA1) lectins. Serial sections were pretreated with trypsin or neuraminidase to determine the effect of these enzymes on lectin binding. The lectins were visualized by the peroxidase antiperoxidase technique and the cell staining proportion assessed in a semi-quantitative manner under the light microscope. Correlating staining with prognostic factors and patient follow-up details showed that UEA1 related to disease-free interval and survival, and HPA to lymph node stage, time to loco regional recurrence and to survival. Relationships with both lectins were abolished by pretreatment with neuraminidase. The study demonstrates that a simple assessment of lectin binding can provide prognostic information in breast cancer. This may be useful particularly when conservational surgical practice restricts the amount of nodal tissue for staging.
对100例连续乳腺癌病例的福尔马林固定、石蜡包埋组织进行了与罗马蜗牛（HPA）和荆豆（UEA1）凝集素结合的研究。连续切片用胰蛋白酶或神经氨酸酶预处理，以确定这些酶对凝集素结合的影响。通过过氧化物酶抗过氧化物酶技术使凝集素可视化，并在光学显微镜下以半定量方式评估细胞染色比例。将染色与预后因素和患者随访细节相关联显示，UEA1与无病间期和生存率相关，而HPA与淋巴结分期、局部区域复发时间和生存率相关。用神经氨酸酶预处理可消除与两种凝集素的关系。该研究表明，简单评估凝集素结合可为乳腺癌提供预后信息。这可能特别有用，尤其是当保乳手术限制了用于分期的淋巴结组织量时。
