Walker R A
J Pathol. 1984 Apr;142(4):279-91. doi: 10.1002/path.1711420406.
Six peroxidase-labelled lectins have been applied to tissue sections of normal, hyperplastic and lactating human breast. The effect of fixation on binding has been assessed by the comparison of specimens treated with a variety of fixatives with frozen material; the effect of trypsin has also been considered. The other objective has been to establish the consistency of lectin reactivity with this non-malignant breast tissue. Fixation has little effect on the binding of wheat germ agglutinin and peanut lectin, although reactivity with the latter was sometimes increased above that seen in frozen material by the use of Bouin's fluid. Trypsinization of formalin-fixed tissue appears to be a prerequisite for staining with soy bean agglutinin and Ulex europeus. The binding of Lotus tetragonolobus and Dolichos biflorus to fixed tissue has been found to be unreliable in comparison to frozen samples. Soy bean agglutinin and Dolichos biflorus, both specific for N-acetyl-D-galactosamine, have binding patterns which vary between specimens and often between themselves. Of the two fucose-binding lectins Ulex europeus has shown a variability of reaction between specimens, whilst Lotus tetragonolobus consistently reacts with ductal and acinar epithelium but with varying intensities. Consistent binding patterns have been found for wheat germ agglutinin and peanut lectin, the latter after the use of neuraminidase. They therefore form a useful basis for comparison with the reactivity of malignant breast tissue.
六种过氧化物酶标记的凝集素已应用于正常、增生和哺乳期人类乳腺的组织切片。通过将用各种固定剂处理的标本与冷冻材料进行比较，评估了固定对结合的影响；还考虑了胰蛋白酶的作用。另一个目标是确定凝集素与这种非恶性乳腺组织反应性的一致性。固定对麦胚凝集素和花生凝集素的结合影响很小，尽管使用波因氏液有时会使后者与冷冻材料相比的反应性增加。福尔马林固定组织的胰蛋白酶消化似乎是用大豆凝集素和欧洲荆豆凝集素染色的先决条件。与冷冻样品相比，已发现四角豆凝集素和双花扁豆凝集素与固定组织的结合不可靠。大豆凝集素和双花扁豆凝集素都对N-乙酰-D-半乳糖胺具有特异性，它们的结合模式在标本之间以及它们自身之间经常有所不同。在两种岩藻糖结合凝集素中，欧洲荆豆在标本之间显示出反应的变异性，而四角豆凝集素始终与导管和腺泡上皮反应，但强度不同。已发现麦胚凝集素和花生凝集素具有一致的结合模式，后者在使用神经氨酸酶后。因此，它们构成了与恶性乳腺组织反应性进行比较的有用基础。
