Corbu Viorica Maria, Dumbravă Andreea Ștefania, Gheorghe-Barbu Irina, Csutak Ortansa
Department of Genetics, Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, 060101 Bucharest, Romania.
Research Institute of University of Bucharest (ICUB), University of Bucharest, B.P. Hasdeu Street 7, 050568 Bucharest, Romania.
Life (Basel). 2024 Aug 29;14(9):1087. doi: 10.3390/life14091087.
Epiphytic yeasts represent an important source for the development of novel strategies aiming to combat food microbial contamination. The present study deals with the characterization of nine yeast strains belonging to , , , , and genera, isolated from the surface of plants from the Botanical Garden "Dimitrie Brandza" (Bucharest, Romania) for use as antimicrobial and probiotic agents. The tests involved the determination of the safe status, cell growth under stress conditions, and activity against pathogenic and bacteria strains, respectively, as well as phytopathogenic filamentous fungi and lipolytic activity. None of the nine strains showed all the characteristics for virulence and pathogenicity, with the rare positive results being explained rather by their adaptability to the habitats of origin. The strains CMGB-ST12 and CMGB-ST19 grew at 37 °C; CMGB-ST21.2, CMGB-ST.8.1 and CMGB ST10 grew in the presence of 10% NaCl, while CMGB-ST12 and CMGB-ST19 tolerated both acidic and alkaline pH values well (3.0 to 12.0). The studied yeast strains showed good antimicrobial activity against , and Gram-negative bacterial strains, with CMGB-ST19 and CMGB-ST53 inhibiting up to 100% the development of filamentous fungi. All the strains produced lipases for tributyrin hydrolysis, the best producer being CMGB-ST1, and only CMGB-ST8.2 tested positive against other probiotic yeasts. Overall, our nine yeast strains show high potential for industrial applications, for obtaining probiotic products and for preventing the development of a wide range of microbial food contaminants.
附生酵母是开发旨在对抗食品微生物污染的新策略的重要来源。本研究对从“迪米特里·布兰扎”植物园（罗马尼亚布加勒斯特）的植物表面分离出的属于、、、、和属的9株酵母菌株进行了表征，以用作抗菌剂和益生菌剂。测试分别涉及确定安全状态、应激条件下的细胞生长以及对致病性和细菌菌株的活性，以及植物病原丝状真菌和脂肪分解活性。这9株菌株均未表现出所有的毒力和致病性特征，罕见的阳性结果更多是由于它们对原生栖息地的适应性。CMGB - ST12和CMGB - ST19菌株能在37℃生长；CMGB - ST21.2、CMGB - ST.8.1和CMGB ST10能在10% NaCl存在的情况下生长，而CMGB - ST12和CMGB - ST19对酸性和碱性pH值（3.0至12.0）都有很好的耐受性。所研究的酵母菌株对、和革兰氏阴性细菌菌株表现出良好的抗菌活性，CMGB - ST19和CMGB - ST53对丝状真菌的生长抑制率高达100%。所有菌株都产生用于水解三丁酸甘油酯的脂肪酶，最佳生产者是CMGB - ST1，只有CMGB - ST8.2对其他益生菌酵母测试呈阳性。总体而言，我们的9株酵母菌株在工业应用、获得益生菌产品以及预防多种微生物食品污染物的生长方面显示出很高的潜力。
