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直接接触矿物三氧化物聚合体的活牙髓细胞的特征。

Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate.

机构信息

Department of Endodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.

Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.

出版信息

Cells. 2020 Oct 21;9(10):2336. doi: 10.3390/cells9102336.

DOI:10.3390/cells9102336
PMID:33096862
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7589724/
Abstract

Mineral trioxide aggregate (MTA) was introduced as a material for dental endodontic regenerative therapy. Here, we show the dynamics of living dental pulp cells in direct contact with an MTA disk. A red fluorescence protein (DsRed) was introduced into immortalized porcine dental pulp cells (PPU7) and cloned. DsRed-PPU7 cells were cultured on the MTA disk and cell proliferation, chemotaxis, the effects of growth factors and the gene expression of cells were investigated at the biological, histomorphological and genetic cell levels. Mineralized precipitates formed in the DsRed-PPU7 cells were characterized with crystal structural analysis. DsRed-PPU7 cells proliferated in the central part of the MTA disk until Day 6 and displayed a tendency to move to the outer circumference. Both transforming growth factor beta and bone morphogenetic protein promoted the proliferation and movement of DsRed-PPU7 cells and also enhanced the expression levels of odontoblastic gene differentiation markers. Mineralized precipitates formed in DsRed-PPU7 were composed of calcium and phosphate but its crystals were different in each position. Our investigation showed that DsRed-PPU7 cells in direct contact with the MTA disk could differentiate into odontoblasts by controlling cell-cell and cell-substrate interactions depending on cell adhesion and the surrounding environment of the MTA.

摘要

矿化三氧化物凝聚体（MTA）被引入作为牙髓再生治疗的牙科材料。在这里，我们展示了与 MTA 盘直接接触的活牙髓细胞的动力学。红色荧光蛋白（DsRed）被引入到永生化猪牙髓细胞（PPU7）中并进行了克隆。DsRed-PPU7 细胞在 MTA 盘上培养，并在生物学、组织形态学和遗传细胞水平上研究了细胞增殖、趋化性、生长因子的作用和基因表达。用晶体结构分析对 DsRed-PPU7 细胞中的矿化沉淀物进行了表征。DsRed-PPU7 细胞在 MTA 盘的中心部分增殖，直到第 6 天，并显示出向外周移动的趋势。转化生长因子β和骨形态发生蛋白都促进了 DsRed-PPU7 细胞的增殖和迁移，并增强了牙本质基因分化标志物的表达水平。DsRed-PPU7 中形成的矿化沉淀物由钙和磷组成，但晶体在每个位置都不同。我们的研究表明，与 MTA 盘直接接触的 DsRed-PPU7 细胞可以通过控制细胞-细胞和细胞-基质相互作用，根据细胞黏附和 MTA 周围环境分化为成牙本质细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/a95022cbd924/cells-09-02336-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/2b4d73146052/cells-09-02336-g0A1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/cc8b2526decf/cells-09-02336-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/5f8eaf491994/cells-09-02336-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/de7982b0706e/cells-09-02336-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/dff434cc42bb/cells-09-02336-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/6ba922427009/cells-09-02336-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/3c4af8a50fb1/cells-09-02336-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/76099022eab9/cells-09-02336-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/11ef34e91c3f/cells-09-02336-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/a95022cbd924/cells-09-02336-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/2b4d73146052/cells-09-02336-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/8d7038dc9eff/cells-09-02336-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/cfa394dda8ee/cells-09-02336-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/cc8b2526decf/cells-09-02336-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/5f8eaf491994/cells-09-02336-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/de7982b0706e/cells-09-02336-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/dff434cc42bb/cells-09-02336-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/6ba922427009/cells-09-02336-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/3c4af8a50fb1/cells-09-02336-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/76099022eab9/cells-09-02336-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/11ef34e91c3f/cells-09-02336-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/7589724/a95022cbd924/cells-09-02336-g010.jpg

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