Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA
Development. 2020 Oct 23;147(20):dev193193. doi: 10.1242/dev.193193.
Since its discovery 150 years ago, the neural crest has intrigued investigators owing to its remarkable developmental potential and extensive migratory ability. Cell lineage analysis has been an essential tool for exploring neural crest cell fate and migration routes. By marking progenitor cells, one can observe their subsequent locations and the cell types into which they differentiate. Here, we review major discoveries in neural crest lineage tracing from a historical perspective. We discuss how advancing technologies have refined lineage-tracing studies, and how clonal analysis can be applied to questions regarding multipotency. We also highlight how effective progenitor cell tracing, when combined with recently developed molecular and imaging tools, such as single-cell transcriptomics, single-molecule fluorescence hybridization and high-resolution imaging, can extend the scope of neural crest lineage studies beyond development to regeneration and cancer initiation.
自 150 年前被发现以来，神经嵴因其显著的发育潜力和广泛的迁移能力而引起了研究人员的兴趣。细胞谱系分析一直是探索神经嵴细胞命运和迁移途径的重要工具。通过标记祖细胞，可以观察它们随后的位置以及它们分化成的细胞类型。在这里，我们从历史的角度回顾了神经嵴谱系追踪的主要发现。我们讨论了先进技术如何改进谱系追踪研究，以及克隆分析如何应用于关于多能性的问题。我们还强调了有效的祖细胞追踪，当与最近开发的分子和成像工具（如单细胞转录组学、单分子荧光杂交和高分辨率成像）结合使用时，如何将神经嵴谱系研究的范围从发育扩展到再生和癌症起始。
