Zhou Yan, Snead Malcolm L
University of Southern California School of Dentistry, Center for Craniofacial Molecular Biology, 2250 Alcazar Street, CSA Room 103, Los Angeles, CA 90033, USA.
Biochem Biophys Res Commun. 2008 Nov 21;376(3):542-7. doi: 10.1016/j.bbrc.2008.09.032. Epub 2008 Sep 18.
The neural crest is a transient population of multipotent progenitors contributing to a diverse array of tissues throughout the vertebrate embryo. Embryonic stem (ES) cells are able to form embryoid body and spontaneously differentiate to various lineages, following a reproducible temporal pattern of development that recapitulates early embryogenesis. Embryoid bodies were triturated and the dissociated cells were processed for fluorescence-activated cell sorting (FACS), and more than 1% of cells were identified as frizzled-3(+)/cadherin-11(+). Expression of marker genes associated with various terminal fates was detected for chondrocytes, glia, neurons, osteoblasts and smooth muscles, indicating that the FACS-sorted frizzled-3(+)/cadherin-11(+) cells were multipotent progenitor cells capable of differentiating to fates associated with cranial neural crest. Moreover, the sorted cells were able to self-renew and maintain multipotent differentiation potential. The derivation of cranial neural crest-like multipotent progenitor cells from ES cells provides a new tool for cell lineage analysis of neural crest in vitro.
神经嵴是一群短暂存在的多能祖细胞，对整个脊椎动物胚胎的多种组织都有贡献。胚胎干细胞（ES细胞）能够形成胚状体并自发分化为各种谱系，遵循一种可重复的时间发育模式，概括了早期胚胎发生过程。将胚状体研磨，对解离的细胞进行荧光激活细胞分选（FACS），超过1%的细胞被鉴定为卷曲蛋白-3（+）/钙黏蛋白-11（+）。检测了与各种终末命运相关的标记基因在软骨细胞、神经胶质细胞、神经元、成骨细胞和平滑肌中的表达，表明经FACS分选的卷曲蛋白-3（+）/钙黏蛋白-11（+）细胞是能够分化为与颅神经嵴相关命运的多能祖细胞。此外，分选的细胞能够自我更新并维持多能分化潜能。从ES细胞中获得颅神经嵴样多能祖细胞为体外神经嵴细胞谱系分析提供了一种新工具。
