Department of Molecular Microbiology and the Rappaport Institute for Medical Sciences, Faculty of Medicine, The Technion-Israel Institute of Technology, Haifa, Israel.
CNRS, Molecular Microbiology and Structural Biochemistry (MMSB, UMR 5086), University of Lyon, Lyon, France.
FEBS Lett. 2020 Dec;594(23):3799-3814. doi: 10.1002/1873-3468.13966. Epub 2020 Nov 21.
In the past two decades, the ATP-binding cassette (ABC) transporters' field has undergone a structural revolution. The importance of structural biology to the development of the field of ABC transporters cannot be overstated, as the ensemble of structures not only revealed the architecture of ABC transporters but also shaped our mechanistic view of these remarkable molecular machines. Nevertheless, we advocate that the mechanistic interpretation of the structures is not trivial and should be carried out with prudence. Herein, we bring several examples of structures of ABC transporters that merit re-interpretation via careful comparison to experimental data. We propose that it is of the upmost importance to place new structures within the context of the available experimental data.
在过去的二十年中，ATP 结合盒（ABC）转运蛋白领域经历了一场结构革命。结构生物学对 ABC 转运蛋白领域的发展具有至关重要的意义，因为这些结构不仅揭示了 ABC 转运蛋白的结构，还塑造了我们对这些非凡的分子机器的机械观点。然而，我们认为对结构的机械解释并非微不足道，应该谨慎进行。在这里，我们通过与实验数据的仔细比较，提出了几个值得重新解释的 ABC 转运蛋白结构的例子。我们建议，将新结构置于现有实验数据的背景下是非常重要的。
