Allen R D, Bernier F, Lessard P A, Beachy R N
Department of Biology, Washington University, Saint Louis, Missouri 63130.
Plant Cell. 1989 Jun;1(6):623-31. doi: 10.1105/tpc.1.6.623.
Upstream sequences of the gene encoding the alpha' subunit of beta-conglycinin were analyzed for interactions with nuclear proteins from immature soybean seeds. Two factors were identified that interact with specific sequence elements within 257 base pairs 5' of the transcription start site. One factor, SEF 3, binds exclusively to a region composed of two elements located at -183 to -169 base pairs and -153 to -134 base pairs relative to the start of transcription. Each of these sites includes the hexanucleotide sequence AACCCA, which may serve as a primary recognition sequence. During seed development, SEF 3 binding activity was found to increase in soybean embryos during the time of beta-conglycinin synthesis and to decrease as seeds neared maturity. The position of the SEF 3 binding sequence corresponds with a previously reported seed-specific enhancer region, and it seems likely that this factor may act as a positive regulator of transcription of the beta-conglycinin, alpha' subunit gene in developing soybean seeds. The second factor, SEF 4, also binds within the -257 to -77 region but also interacts with sites located further upstream.
对编码β-伴大豆球蛋白α’亚基的基因上游序列进行分析，以研究其与未成熟大豆种子核蛋白的相互作用。鉴定出两个与转录起始位点5’端257个碱基对内特定序列元件相互作用的因子。其中一个因子SEF 3专门结合到一个由两个元件组成的区域，这两个元件相对于转录起始点位于-183至-169碱基对以及-153至-134碱基对处。这些位点中的每一个都包含六核苷酸序列AACCCA，它可能作为主要识别序列。在种子发育过程中，发现SEF 3结合活性在β-伴大豆球蛋白合成期间在大豆胚中增加，并在种子接近成熟时降低。SEF 3结合序列的位置与先前报道的种子特异性增强子区域相对应，并且这个因子似乎可能作为发育中的大豆种子中β-伴大豆球蛋白α’亚基基因转录的正调控因子。第二个因子SEF 4也结合在-257至-77区域内，但也与更上游的位点相互作用。
