Kuzmin Petr, Bukharina Irina, Kuzmina Ajgul
Department of Biology and Chemistry, Yelabuga Institute of Kazan Federal University, Kazan str., 89, Yelabuga 423600, Russian Federation.
Institute of Civil Protection, Udmurt State University, Universitetskaya str., 1, Izhevsk 426069, Russian Federation.
Saudi J Biol Sci. 2020 Nov;27(11):2912-2916. doi: 10.1016/j.sjbs.2020.09.010. Epub 2020 Sep 12.
The study, which covers the period between 2014 and 2018, was carried out in the city of Naberezhnye Chelny, Republic of Tatarstan, Russia. The aim of the study was to examine the biochemical response of maple trees growing in the anthropogenic environments. Leaf samples from 600 trees ( L. and L.) were collected at monthly intervals from June through August. Sampling was performed early in the morning (11 a.m.) in the middle of the month. The study offers statistical data on the tannin content, determined via permanganometry; the ascorbic acid concentration, found via titration with 2.6-dichlorophenolindophenol; the ascorbate oxidase activity determined by absorbance at 265 nm; and the polyphenol oxidase activity, found by the spectrophotometric method. Relatively higher ascorbate oxidase activity was detected in August among ash-leaved L. and L. in areas with strong anthropogenic impact. Due to increased air pollution, maple trees were found to exhibit an increase of polyphenol oxidase activities. The condensed tannin content in Norway maple trees dropped over time: by 1.24 in July (avenue); by 0.94 (buffer area) and 0.76 (avenue) in August. The condensed tannin content in the ash-leaved maple trees also decreased: by 0.69 (buffer area) and 0.22 (avenue) in July; by 0.37 (buffer area) and 0.61(avenue) in August.
该研究涵盖2014年至2018年期间，在俄罗斯鞑靼斯坦共和国下卡姆斯克市开展。研究目的是检测生长在人为环境中的枫树的生化反应。从600棵树（白蜡槭和挪威槭）上每月采集一次叶片样本，采集时间为6月至8月。采样于每月中旬上午11点进行。该研究提供了通过高锰酸钾滴定法测定的单宁含量、通过2,6-二氯酚靛酚滴定法测定的抗坏血酸浓度、通过265nm吸光度测定的抗坏血酸氧化酶活性以及通过分光光度法测定的多酚氧化酶活性的统计数据。在8月，受强烈人为影响地区的灰叶白蜡槭和挪威槭中检测到相对较高的抗坏血酸氧化酶活性。由于空气污染加剧，发现枫树的多酚氧化酶活性增加。挪威槭中的缩合单宁含量随时间下降：7月下降1.24（林荫道）；8月下降0.94（缓冲区）和0.76（林荫道）。灰叶槭中的缩合单宁含量也下降：7月下降0.69（缓冲区）和0.22（林荫道）；8月下降0.37（缓冲区）和0.61（林荫道）。
