Zhang Shuang, Taehwan Kim Aaron, Liu Xiaoyong, Yan Lei, Moo Kim Sang
Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, People's Republic of China.
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Republic of Korea.
J Food Biochem. 2020 Dec;44(12):e13540. doi: 10.1111/jfbc.13540. Epub 2020 Oct 25.
The antioxidant and antidiabetic activities of vanadium-binding protein (VBP) and trifuhalol A, alone or combined, were investigated. Both VBP and trifuhalol A showed potent radical scavenging activity (RSA) on 2,2'-azinobis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), hydrogen peroxide, and ferric-reducing antioxidant power. Their combination at a concentration of 100 μg/ml VBP and 40 μg/ml trifuhalol A exhibited more than 99% RSA against ABTS. Additionally, VBP and trifuhalol A, alone or combined, displayed potential antidiabetic activities against Saccharomyces cerevisiae α-glucosidase. The highest inhibition of 70.26% against S. cerevisiae α-glucosidase was observed in the case of the combination of 250 μg/ml VBP and 1.75 μg/ml trifuhalol A. Kinetics study revealed that VBP and trifuhalol A were noncompetitive inhibition type against S. cerevisiae α-glucosidase, while VBP and trifuhalol A combined treatment was a mixed inhibition type against S. cerevisiae α-glucosidase. These results indicated that VBP and trifuhalol A, alone or combined, had high free radical scavenging activity and inhibitory activity against S. cerevisiae a-glucosidase, suggesting that VBP and trifuhalol A could be used as candidates for the development of natural antidiabetic drugs or functional food. PRACTICAL APPLICATIONS: The present study showed that VBP and trifuhalol A, alone or combined, had potential antioxidant and antidiabetic activities, suggesting that VBP and trifuhalol A could be developed to a novel nutraceutical or natural antidiabetic drugs in the management of obesity or diabetes. This finding will be beneficial for all peoples who are directly or indirectly associated with obesity or diabetes.
研究了钒结合蛋白（VBP）和三氟哈洛醇A单独或联合使用时的抗氧化和抗糖尿病活性。VBP和三氟哈洛醇A对2,2'-偶氮二（3-乙基苯并噻唑啉）-6-磺酸（ABTS）、过氧化氢和铁还原抗氧化能力均表现出较强的自由基清除活性（RSA）。它们以100μg/ml VBP和40μg/ml三氟哈洛醇A的浓度组合时，对ABTS的RSA超过99%。此外，VBP和三氟哈洛醇A单独或联合使用时，对酿酒酵母α-葡萄糖苷酶均显示出潜在的抗糖尿病活性。在250μg/ml VBP和1.75μg/ml三氟哈洛醇A组合的情况下，观察到对酿酒酵母α-葡萄糖苷酶的最高抑制率为70.26%。动力学研究表明，VBP和三氟哈洛醇A对酿酒酵母α-葡萄糖苷酶为非竞争性抑制类型，而VBP和三氟哈洛醇A联合处理对酿酒酵母α-葡萄糖苷酶为混合型抑制类型。这些结果表明，VBP和三氟哈洛醇A单独或联合使用时，对酿酒酵母α-葡萄糖苷酶具有较高的自由基清除活性和抑制活性，表明VBP和三氟哈洛醇A可作为天然抗糖尿病药物或功能性食品开发的候选物。实际应用：本研究表明，VBP和三氟哈洛醇A单独或联合使用具有潜在的抗氧化和抗糖尿病活性，表明VBP和三氟哈洛醇A可开发为新型营养保健品或天然抗糖尿病药物，用于肥胖或糖尿病的管理。这一发现将对所有直接或间接与肥胖或糖尿病相关的人群有益。
