Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, P.O. Box: 76135-133, Kerman, Iran.
Laboratory of Molecular Neuroscience, Kerman Neuroscience Research Center (KNRC), Kerman University of Medical Sciences, Kerman, Iran.
Exp Brain Res. 2021 Jan;239(1):79-93. doi: 10.1007/s00221-020-05961-1. Epub 2020 Oct 26.
The amygdala has been demonstrated to contribute to pain-related behavior and food preference. Here, the effect of pain on food preference and food-matched visual-cue memory, in the presence or absence of a basolateral amygdala (BLA) lesion, has been evaluated using a novel innovative apparatus and protocol. Forty adult male Wistar rats were randomly divided into five groups (n = 8) as follows: control, pain, ibuprofen + pain, BLA lesion, BLA lesion + pain groups. Bilateral lesions of the BLA were produced by passing a current of 1.5 mA for 7 s. Pain was induced on the right hind paw of the rats by sub-plantar injection of 50 μl of 2.5% formalin. The animals were encountered with four different meals including wholemeal, wholemeal + sugar, white flour, and biscuit. Each test session consisted of six trials with inter-trial intervals of 15 min. The number of visits to each meal zone and port, the amount of time spent in each food zone and port, traveled distance in each food zone, food consumption per each visit and the total food consumption were recorded. The control group showed a high biscuit preference and low white flour preference. Rats suffering BLA lesion and rats in the BLA lesion + pain group exhibited a shifted preference curve. They had a bias toward eating wholemeal + sugar rather than white flour and biscuit. This group also showed an impaired spatial memory. In conclusion, our findings suggest that the BLA may be involved in pain-induced food preference and food-matched visual-cue memory.
杏仁核已被证明有助于与疼痛相关的行为和食物偏好。在这里，使用一种新颖的创新仪器和方案评估了疼痛对食物偏好和食物匹配视觉线索记忆的影响，同时存在或不存在基底外侧杏仁核（BLA）损伤。40 只成年雄性 Wistar 大鼠被随机分为 5 组（n=8）：对照组、疼痛组、布洛芬+疼痛组、BLA 损伤组、BLA 损伤+疼痛组。通过传递 1.5 mA 的电流 7 s 来产生双侧 BLA 损伤。通过在大鼠右后脚掌注射 50 μl 2.5%甲醛来诱导疼痛。动物遇到四种不同的餐食，包括全麦、全麦+糖、白面粉和饼干。每次测试包括 6 次试验，试验间隔 15 分钟。记录访问每个膳食区和端口的次数、在每个食物区和端口花费的时间、在每个食物区的行驶距离、每次访问的食物摄入量和总食物摄入量。对照组表现出高饼干偏好和低白面粉偏好。BLA 损伤大鼠和 BLA 损伤+疼痛组大鼠表现出偏好曲线的转移。他们倾向于吃全麦+糖而不是白面粉和饼干。该组还表现出空间记忆受损。总之，我们的研究结果表明，BLA 可能参与了疼痛引起的食物偏好和食物匹配视觉线索记忆。
