Tagliamonte Silvia, Gill Chris I R, Pourshahidi L Kirsty, Slevin Mary M, Price Ruth K, Ferracane Rosalia, Lawther Roger, O'Connor Gloria, Vitaglione Paola
Department of Agricultural Sciences, University of Naples "Federico II", Via Università 100, 80055, Portici, NA, Italy.
Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine, UK.
Eur J Nutr. 2021 Jun;60(4):2203-2215. doi: 10.1007/s00394-020-02398-8. Epub 2020 Oct 26.
To determine the small intestinal concentration of endocannabinoids (ECs), N-acylethanolamines (NAEs) and their precursors N-acylphosphatidylethanolamines (NAPEs) in humans. To identify relationships between those concentrations and habitual diet composition as well as individual inflammatory status.
An observational study was performed involving 35 participants with an ileostomy (18W/17M, aged 18-70 years, BMI 17-40 kg/m). Overnight fasting samples of ileal fluid and plasma were collected and ECs, NAEs and NAPEs concentrations were determined by LC-HRMS. Dietary data were estimated from self-reported 4-day food diaries.
Regarding ECs, N-arachidonoylethanolamide (AEA) was not detected in ileal fluids while 2-arachidonoylglycerol (2-AG) was identified in samples from two participants with a maximum concentration of 129.3 µg/mL. In contrast, mean plasma concentration of AEA was 2.1 ± 0.06 ng/mL and 2-AG was 4.9 ± 1.05 ng/mL. NAEs concentrations were in the range 0.72-17.6 µg/mL in ileal fluids and 0.014-0.039 µg/mL in plasma. NAPEs concentrations were in the range 0.3-71.5 µg/mL in ileal fluids and 0.19-1.24 µg/mL in plasma being more abundant in participants with obesity than normal weight and overweight. Significant correlations between the concentrations of AEA, OEA and LEA in biological fluids with habitual energy or fat intakes were identified. Plasma PEA positively correlated with serum C-reactive protein.
We quantified ECs, NAEs and NAPEs in the intestinal lumen. Fat and energy intake may influence plasma and intestinal concentrations of these compounds. The luminal concentrations reported would allow modulation of the homeostatic control of food intake via activation of GPR119 receptors located on the gastro-intestinal mucosa.
NCT04143139; www.clinicaltrials.gov .
测定人体内小肠中内源性大麻素（ECs）、N-酰基乙醇胺（NAEs）及其前体N-酰基磷脂酰乙醇胺（NAPEs）的浓度。确定这些浓度与习惯性饮食组成以及个体炎症状态之间的关系。
进行了一项观察性研究，纳入35名回肠造口术参与者（18名女性/17名男性，年龄18 - 70岁，BMI 17 - 40kg/m²）。收集过夜禁食后的回肠液和血浆样本，通过液相色谱-高分辨质谱法测定ECs、NAEs和NAPEs的浓度。饮食数据通过自我报告的4天食物日记估算。
关于ECs，在回肠液中未检测到N-花生四烯酰乙醇胺（AEA），而在两名参与者的样本中鉴定出2-花生四烯酰甘油（2-AG），最高浓度为129.3μg/mL。相比之下，AEA的平均血浆浓度为2.1±0.06ng/mL，2-AG为4.9±1.05ng/mL。NAEs在回肠液中的浓度范围为0.72 - 17.6μg/mL，在血浆中为0.014 - 0.039μg/mL。NAPEs在回肠液中的浓度范围为0.3 - 71.5μg/mL，在血浆中为0.19 - 1.24μg/mL，肥胖参与者比正常体重和超重参与者更为丰富。在生物体液中，AEA、OEA和LEA的浓度与习惯性能量或脂肪摄入量之间存在显著相关性。血浆中PEA与血清C反应蛋白呈正相关。
我们对肠腔内的ECs、NAEs和NAPEs进行了定量。脂肪和能量摄入可能会影响这些化合物在血浆和肠道中的浓度。所报告的肠腔浓度可能通过激活位于胃肠道黏膜上的GPR119受体来调节食物摄入的稳态控制。
NCT04143139；www.clinicaltrials.gov 。
