Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, Brazil.
Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Braz J Microbiol. 2020 Dec;51(4):1509-1513. doi: 10.1007/s42770-020-00386-y. Epub 2020 Oct 26.
Panton-Valentine leukocidin (PVL) is a Staphylococcus aureus virulence factor codified by lukSF-PV genes. Single-nucleotide polymorphisms (SNPs) at lukSF-PV genes can lead to two PVL sequence variants (R and H) generating different PVL isoforms. This study analyzed lukSF-PV genes SNPs among four different clonal lineages (STs/CC 1, 5, 8, and 30) of nine S. aureus isolated at Brazilian hospitals. The sequenced products showed SNPs at seven sites (positions 121, 470, 527, 663, 856, 1396, and 1729), leading to non-synonymous substitutions in all isolates investigated. Our findings showed new R and H isoforms variants in S. aureus isolated in Brazil and suggest a possible relationship between H2b isoform and the ST30/CC30 lineage.
Panton-Valentine 白细胞毒素（PVL）是编码金黄色葡萄球菌毒力因子的 lukSF-PV 基因。lukSF-PV 基因中的单核苷酸多态性（SNPs）可导致两种 PVL 序列变异体（R 和 H）产生不同的 PVL 同工型。本研究分析了巴西医院分离的 9 株金黄色葡萄球菌的四个不同克隆谱系（ST/CC1、5、8 和 30）中 lukSF-PV 基因的 SNPs。测序产物显示在七个位置（位置 121、470、527、663、856、1396 和 1729）存在 SNPs，导致所有研究的分离株均发生非同义取代。我们的研究结果显示了巴西分离的金黄色葡萄球菌中出现了新的 R 和 H 同工型变体，并提示 H2b 同工型与 ST30/CC30 谱系之间可能存在关联。
