Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Biology, Reed College, Portland, OR, USA.
Sci Data. 2020 Oct 26;7(1):367. doi: 10.1038/s41597-020-00713-w.
Bumblebees (Hymenoptera: Apidae) are important pollinating insects that play pivotal roles in crop production and natural ecosystem services. Although protein-coding genes in bumblebees have been extensively annotated, regulatory sequences of the genome, such as promoters and enhancers, have been poorly annotated. To achieve a comprehensive profile of accessible chromatin regions and provide clues for all possible regulatory elements in the bumblebee genome, we performed ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) on Bombus terrestris samples derived from four developmental stages: egg, larva, pupa, and adult, respectively. The ATAC-seq reads were mapped to the B. terrestris reference genome, and its accessible chromatin regions were identified and characterized using bioinformatic methods. We identified 36,390 chromatin accessible regions in total, including both shared and stage-specific chromatin accessible signals. Our study will provide an important resource, not only for uncovering regulatory elements in the bumblebee genome, but also for expanding our understanding of bumblebee biology throughout development.
熊蜂（膜翅目：蜜蜂科）是重要的传粉昆虫，在作物生产和自然生态系统服务中发挥着关键作用。尽管熊蜂的蛋白质编码基因已被广泛注释，但基因组的调控序列，如启动子和增强子，注释很差。为了全面描绘可及染色质区域，并为熊蜂基因组中的所有可能的调控元件提供线索，我们对分别来自卵、幼虫、蛹和成虫四个发育阶段的熊蜂样本进行了 ATAC-seq（高通量测序的转座酶可及染色质分析）实验。将 ATAC-seq 读取序列映射到熊蜂参考基因组上，并使用生物信息学方法鉴定和描述其可及染色质区域。我们总共鉴定了 36390 个染色质可及区域，包括共享和阶段特异性的染色质可及信号。我们的研究不仅为揭示熊蜂基因组中的调控元件提供了重要资源，也为我们在整个发育过程中扩展对熊蜂生物学的理解提供了重要资源。
