Beasi W R, Toffoli L V, Pelosi G G, Gomes M V M, Verissimo L F, Stocco M R, Mantoani L C, Maia L P, Andraus R A C
Program Stricto Sensu in Rehabilitation Sciences, Research and Postgraduate Center - UNOPAR/UEL, Londrina, Brazil.
Department of Physiological Sciences, Center of Biological Sciences, State University of Londrina - UEL, Londrina, Brazil.
Lasers Med Sci. 2021 Sep;36(7):1379-1387. doi: 10.1007/s10103-020-03168-y. Epub 2020 Oct 27.
The aim of the present study was to evaluate the effects of photobiomodulation (low-level laser therapy (LLLT)) and aquatic exercise on the expression of genes related to muscle regeneration in rats. Wistar rats were divided into five groups: control group (n = 15), non-treated injury group (n = 15), injury+LLLT group (n = 15), injury+aquatic exercise group (n = 15), and injury+LLLT+aquatic exercise group (n = 15). Cryoinjury was performed on the belly of the tibialis anterior (TA) muscle. LLLT was performed daily with an AlGaAs laser (830 nm; beam spot of 0.0324 cm, output power of 100 mW, energy density of 180 J/cm, and 58-s exposure time). Animals were euthanized at 7, 14, and 21 days. The TA muscles were removed for gene expression analysis of TGF-β, Myogenin, and MyoD. The results were statistically analyzed at a significance level of 5%. The cryoinjury increased the expression of genes related to muscle regeneration-MyoD, Myogenin, and TGF-β-compared to the control group (p < 0.05); the photobiomodulation increased the expression of these genes at day 7 (p < 0.05), decreasing until day 21; and the aquatic exercise increases the expression of the three genes over time. When the two treatments were combined, the expression of the analyzed genes also increased over time. In summary, the results of our study suggest that photobiomodulation (LLLT), when applied alone in cryoinjury, is able to increase the gene expression of MyoD, Myogenin, and TGF-β at the acute phase, while when combined with aquatic exercises, there is an increase in expression of these genes specially at the long-term treatment.
本研究的目的是评估光生物调节（低强度激光疗法（LLLT））和水上运动对大鼠肌肉再生相关基因表达的影响。将Wistar大鼠分为五组：对照组（n = 15）、未治疗损伤组（n = 15）、损伤+LLLT组（n = 15）、损伤+水上运动组（n = 15）和损伤+LLLT+水上运动组（n = 15）。对胫骨前肌（TA）腹部进行冷冻损伤。每天用铝镓砷激光（830 nm；光斑为0.0324 cm，输出功率为100 mW，能量密度为180 J/cm，照射时间为58秒）进行LLLT。在第7、14和21天对动物实施安乐死。取出TA肌肉用于TGF-β、肌细胞生成素和肌分化抗原（MyoD）的基因表达分析。结果以5%的显著性水平进行统计学分析。与对照组相比，冷冻损伤增加了肌肉再生相关基因——MyoD、肌细胞生成素和TGF-β的表达（p < 0.05）；光生物调节在第7天增加了这些基因的表达（p < 0.05），到第21天下降；水上运动随着时间推移增加了这三种基因的表达。当两种治疗方法联合使用时，所分析基因的表达也随时间增加。总之，我们的研究结果表明，光生物调节（LLLT）单独应用于冷冻损伤时，能够在急性期增加MyoD、肌细胞生成素和TGF-β的基因表达，而与水上运动联合使用时，特别是在长期治疗中这些基因的表达会增加。
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