ELI-ALPS, ELI-HU Non-Profit Ltd., Szeged, Hungary
ELI-ALPS, ELI-HU Non-Profit Ltd., Szeged, Hungary.
Anticancer Res. 2020 Nov;40(11):6123-6135. doi: 10.21873/anticanres.14633.
BACKGROUND/AIM: The importance of hadron therapy in the cancer management is growing. We aimed to refine the biological effect detection using a vertebrate model.
Embryos at 24 and 72 h postfertilization were irradiated at the entrance plateau and the mid spread-out Bragg peak of a 150 MeV proton beam and with reference photons. Radiation-induced DNA double-strand breaks (DSB) and histopathological changes of the eye, muscles and brain were evaluated; deterioration of specific organs (eye, yolk sac, body) was measured.
More and longer-lasting DSBs occurred in eye and muscle cells due to proton versus photon beams, albeit in different numbers. Edema, necrosis and tissue disorganization, (especially in the eye) were observed. Dose-dependent morphological deteriorations were detected at ≥10 Gy dose levels, with relative biological effectiveness between 0.99±0.07 (length) and 1.12±0.19 (eye).
Quantitative assessment of radiation induced changes in zebrafish embryos proved to be beneficial for the radiobiological characterization of proton beams.
背景/目的：强子疗法在癌症治疗中的重要性日益增加。我们旨在利用脊椎动物模型改进生物效应检测。
在受精后 24 小时和 72 小时，用 150 MeV 质子束的入口平台和扩展布拉格峰以及参考光子对胚胎进行照射。评估辐射诱导的 DNA 双链断裂（DSB）和眼睛、肌肉和大脑的组织病理学变化；测量特定器官（眼睛、卵黄囊、身体）的恶化情况。
与光子束相比，质子束在眼睛和肌肉细胞中产生更多和持续时间更长的 DSB，尽管数量不同。观察到水肿、坏死和组织紊乱（尤其是在眼睛中）。在≥10 Gy 剂量水平下检测到剂量依赖性形态恶化，相对生物效应在 0.99±0.07（长度）和 1.12±0.19（眼睛）之间。
定量评估斑马鱼胚胎辐射诱导的变化被证明有利于质子束的放射生物学特性描述。
