German Cancer Research Center (DKFZ), Department of Translational Immunotherapy, Heidelberg, Germany.
National Center for Tumor Diseases, Heidelberg, Germany.
Anticancer Res. 2020 Nov;40(11):6367-6373. doi: 10.21873/anticanres.14657.
BACKGROUND/AIM: The presence of TLS (tertiary lymphoid structures) is detectable in the microenvironment of human cancers and linked to better patient outcomes. The role of TLS in colorectal cancer liver metastases (CRCLM) is unclear.
Medical records of patients with CRCLM were reviewed (n=33) and corresponding tissue samples (n=21) were obtained. Whole slide imaging analyses on immunohistochemical staining of immune cell infiltrates and TLS were correlated to clinical outcomes (including chemotherapy response) and other parameters.
Neither the size (p=0.6) nor the quantity (p=0.47) of TLS was associated with the clinical course. When considering spatial differences, TLS in the invasive margin (IM) were associated with progression-free survival (p=0.03), while TLS in the LM (liver metastasis) were not. Also, TLS in the IM were associated with the presence of CD3 T cells, which itself was prognostically favorable (p<0.001).
TLS in the IM are associated with good prognosis in CRCLM, but not chemotherapy response.
背景/目的：TLS（三级淋巴结构）存在于人类癌症的微环境中，与更好的患者预后相关。TLS 在结直肠癌肝转移（CRCLM）中的作用尚不清楚。
回顾性分析了 CRCLM 患者的病历（n=33），并获得了相应的组织样本（n=21）。对免疫细胞浸润和 TLS 的免疫组化染色的全切片成像分析与临床结果（包括化疗反应）和其他参数相关。
TLS 的大小（p=0.6）和数量（p=0.47）均与临床过程无关。当考虑空间差异时，浸润边缘（IM）中的 TLS 与无进展生存期（p=0.03）相关，而 LM（肝转移）中的 TLS 则没有。此外，IM 中的 TLS 与 CD3 T 细胞的存在相关，而 CD3 T 细胞本身的预后良好（p<0.001）。
CRCLM 中 IM 中的 TLS 与良好的预后相关，但与化疗反应无关。
